Recombinant Mouse Serpin A9/Centerin Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 5679-PI
Key Product Details
Source
NS0
Accession #
Structure / Form
Monomer
Conjugate
Unconjugated
Applications
Inhibition Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Serpin A9/Centerin protein
Asn26-Met418, with an N-terminal 6-His tag
Accession # Q9D7D2
Asn26-Met418, with an N-terminal 6-His tag
Accession # Q9D7D2
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His
Predicted Molecular Mass
45 kDa
SDS-PAGE
40-50 kDa, reducing conditions
Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <7 nM, measured under the described conditions.
The IC50 value is <7 nM, measured under the described conditions.
Formulation, Preparation and Storage
5679-PI
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Serpin A9/Centerin
Serpin A9, also known as Centerin, is a member of the Serpin superfamily of serine protease inhibitors (1). Serpins are the most broadly distributed superfamily of protease inhibitors. They are known to inhibit serine proteases and some are known to inhibit caspases and papain-like cysteine proteases (1). Recombinant mouse Serpin A9 shows inhibition towards trypsin, thrombin, and plasmin and binds DNA and heparin (2). Its expression is limited to germinal center B cells and lymphoid malignancies with germinal center B-cell maturation (3). New findings suggest that Serpin A9 could be used as a potential diagnostic marker in the recognition of various germinal center derived lymphomas (4).
References
Law, R., et al. (2006) Genome Biol. 7:216.
Paterson, M.A., et al. (2007) Biochem J. 405:489.
Pan, Z., et al. (2003) Am. J. Pathol. 163:135.
Montes-Moreno, S. et al. (2008) Blood. 111:351.
Long Name
Serpin Peptidase Inhibitor, Clade A, Member 9
Alternate Names
Centerin, GCET1, SERPINA11b
Gene Symbol
SERPINA9
UniProt
Additional Serpin A9/Centerin Products
Product Documents for Recombinant Mouse Serpin A9/Centerin Protein, CF
Product Specific Notices for Recombinant Mouse Serpin A9/Centerin Protein, CF
For research use only
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