Recombinant Mouse Slit2 (aa 26-900) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5444-SL

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
5444-SL-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

CHO

Accession #

Q9R1B9

Conjugate

Unconjugated

Applications

Bioactivity

View all Slit2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Slit2 protein
Gln26-Gln900, with a C-terminal 10-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained; Gln26 predicted

Predicted Molecular Mass

99.7 kDa

SDS-PAGE

105-115 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
Recombinant Mouse Slit2 immobilized at 3-12 μg/mL, is able to significantly induce neurite outgrowth.

Reviewed Applications

Read 2 reviews rated 5 using 5444-SL in the following applications:

Formulation, Preparation and Storage

5444-SL
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Slit2

Slit Homolog 2 (Slit2) is a member of the Slit family of secreted extracellular matrix glycoproteins that are best known for their role in axon guidance (1). It is expressed in the developing and adult brain and spinal cord, as well as in fetal lung and kidney, and the adult adrenal gland, thyroid gland, and trachea (1-3). Slit2 is composed of multiple domains including nine EGF-like domains, 20 Leucine-rich repeats (LRRs), one Laminin G-like domain, one C-terminal cysteine knot-like (CTCK) domain, and 4 N-terminal and 4 C-terminal LRR domains (1, 3). Slit2 has a molecular weight of approximately 200 kDa (4). However, proteolytic cleavage between the fifth and sixth EGF-like domains produces a membrane-bound 140 kDa N-terminal protein, termed Slit2-N, and a 55-60 kDa C-terminal fragment, termed Slit2-C (4, 5). Mature mouse Slit2 shares 96% and 98% amino acid sequence identity with the human and rat orthologs, respectively.

Slit2 has been shown to bind to multiple receptors including ROBO-1, -2, -3, and -4, Laminin-1, DAN, Gremlin, and Glypican-1 (1, 6-8). Depending upon the target, Slit2 can promote a number of diverse effects. Slit2 regulates axon guidance by binding to ROBO receptors and initiating axon repulsion (1, 5, 9-12). Slit2 has also been shown to induce growth cone collapse, inhibit oligodendrocyte precursor cell migration, and promote axon elongation, branch formation, and fasciculation (5, 13-16). Additionally, Slit2-N and Slit2-C have been shown to have distinct activities. Slit2-N binds to ROBO-1 and repels motor axon migration, while Slit2-C binds to
Glypican-1 and promotes motor axon migration (5). Outside the nervous system, Slit2 plays a role in a wide range of biological processes including cell adhesion and migration, tumor progression and metastasis, angiogenesis, lymphangiogenesis, HIV-1 replication, platelet function and thrombus formation, and stem cell senescence (1, 8, 17-30).

References

  1. Ypsilanti, A.R. et al. (2010) Development 137:1939.
  2. Itoh, A. et al. (1998) Brain Res. Mol. Brain Res. 62:175.
  3. Holmes, G.P. et al. (1998) Mech. Dev. 79:57.
  4. Chédotal, A. (2007) Adv. Exp. Med. Biol. 621:65.
  5. Nguyen Ba-Charvet, K.T. et al. (2001) J. Neurosci. 21:4281.
  6. Nguyen Ba-Charvet, K.T. et al. (2001) Mol. Cell. Neurosci. 17:1048.
  7. Hagino, S. et al. (2003) Glia 42:130.
  8. Chen, B. et al. (2004) J. Immunol. 173:5914.
  9. Yuan, W. et al. (1999) Dev. Biol. 212:290.
  10. Erskine, L. et al. (2000) J. Neurosci. 20:4975.
  11. Shu, T. et al. (2003) J. Neurosci. 23:8176.
  12. Kim, M. et al. (2014) Neural Dev. 9:17.
  13. Wang, K.H. et al. (1999) Cell 96:771.
  14. Wong, E.V. et al. (2004) J. Neurobiol. 59:66.
  15. Liu, X. et al. (2012) J. Biol. Chem. 287:17503.
  16. Jaworski, A. et al. (2012) Nat. Neurosci. 15:367.
  17. Guan, H. et al. (2003) J. Immunol. 171:6519.
  18. Liu, D. et al. (2006) Circ. Res. 98:480.
  19. Prasad, A. et al. (2007) J. Leukoc. Biol. 82:465.
  20. Tole, S. et al. (2009) J. Leukoc. Biol. 86:1403.
  21. Kim, H.K. et al. (2008) Neoplasia 10:1411.
  22. Prasad, A. et al. (2008) J. Biol. Chem. 283:26624.
  23. Tseng, R.C. et al. (2010) Cancer Res. 70:543.
  24. Alajez, N.M. et al. (2011) Cancer Res. 71:2381.
  25. Zhang, Q.Q. et al. (2015) Oncotarget 6:3123.
  26. Dunaway, C.M. et al. (2011) Mol. Cell. Biol. 31:404.
  27. Yang, X.M. et al. (2010) Biochem. Biophys. Res. Commun. 396:571.
  28. Anand, A.R. et al. (2011) AIDS 25:2105.
  29. Patel, S. et al. (2012) Circulation 126:1385.
  30. Harburg, G. et al. (2014) Stem Cell Reports 3:385.

Long Name

SLIT Homolog 2

Alternate Names

SLIL3

Entrez Gene IDs

9353 (Human); 20563 (Mouse); 360272 (Rat)

Gene Symbol

SLIT2

UniProt

Q9R1B9 (Mouse)

Additional Slit2 Products

Product Documents for Recombinant Mouse Slit2 (aa 26-900) Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse Slit2 (aa 26-900) Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.