Recombinant Mouse SLITRK2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9965-SK

R&D Systems, part of Bio-Techne
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9965-SK-050
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Key Product Details

Source

NS0

Accession #

Q810C0

Conjugate

Unconjugated

Applications

Bioactivity

View all SLITRK2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse SLITRK2 protein
Arg22-Leu622, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg22

Predicted Molecular Mass

69 kDa

SDS-PAGE

86-98 kDa, reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200-400 ng on a nitrocellulose-coated microplate.

Scientific Data Images for Recombinant Mouse SLITRK2 Protein, CF

Recombinant Mouse SLITRK2 Protein SDS-PAGE

Recombinant Mouse SLITRK2 Protein SDS-PAGE

2 μg/lane of Recombinant Mouse SLITRK2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 86-98 kDa respectively.

Formulation, Preparation and Storage

9965-SK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: SLITRK2

SLITRK2 (Slit and Trk-like family member 2) is an approximately 110 kDa type I transmembrane member of the SLITRK family of proteins which contain a slit-like extracellular region and a Trk-like cytoplasmic region (1). The extracellular domain (ECD) of mature mouse SLITRK2 contains 6 leucine rich repeats (LRR) followed by a C-terminal LRR domain, followed by 6 more LRR flanked by a pair of N- and C-terminal LRR domains (2, 3). Within the ECD, mouse SLITRK2 shares 98% and 99% amino acid sequence identity with human and rat SLITRK2, respectively. SLITRK2 is expressed in multiple regions of the brain, particularly the cerebral cortex and hippocampus (4). It can suppress neurite outgrowth and promote the formation of excitatory and inhibitory presynaptic structures (3-5). The synaptogenic function is dependent on the interaction of SLITRK2 with select isoforms of PTP-sigma (5, 6). In humans, mutations of SLITRK2 are associated with bipolar disorder and schizophrenia (7, 8).

References

  1. Ko, J. (2012) Mol. Cells 34:335.
  2. Aruga, J. et al. (2003) Gene 315:87.
  3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.
  4. Yim, Y.S. et al. (2013) Proc. Natl. Acad. Sci. USA 110:4057.
  5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
  6. Yamagata, A. et al. (2015) Sci. Rep. 5:9686.
  7. Smith, E.N. et al. (2009) Mol. Pschiatry 14:755.
  8. Piton, A. et al. (2011) Mol. Pschiatry 16:867.

Long Name

SLIT and NTRK-like Family, Member 2

Alternate Names

CXorf2, SLITL1

Entrez Gene IDs

84631 (Human); 245450 (Mouse)

Gene Symbol

SLITRK2

UniProt

Q810C0

Additional SLITRK2 Products

Product Documents for Recombinant Mouse SLITRK2 Protein, CF

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Product Specific Notices for Recombinant Mouse SLITRK2 Protein, CF

For research use only

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