Recombinant Mouse SLITRK5 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9520-SK

R&D Systems, part of Bio-Techne
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9520-SK-050
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Key Product Details

Source

NS0

Accession #

Q810B7

Conjugate

Unconjugated

Applications

Bioactivity

View all SLITRK5 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse SLITRK5 protein
Ile41-Ser664, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile41

Predicted Molecular Mass

70 kDa

SDS-PAGE

96-113 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
Able to significantly enhance neurite outgrowth when immobilized at 3-6 µg/mL.

Formulation, Preparation and Storage

9520-SK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLITRK5

SLITRK5 (Slit and Trk-like family member 5; also known as LRR-containing protein 11) is a member of the SLITRK family of proteins (1, 2). This family currently includes six members, all of which contain a slit-like extracellular region and a Trk-like cytoplasmic region (3). Mouse SLITRK5 is a type I transmembrane (TM) protein that is 957 amino acids (aa) in length. It contains a putative 40 aa signal sequence, a 624 aa extracellular domain (ECD), a 21 aa TM segment, and a 272 aa cytoplasmic region. The ECD is characterized by the presence of two leucine-rich domains (LRD) that resemble those found in SLITs. Each LRD  contains seven and eight leucine-rich repeats (LRRs), respectively. Mouse SLITRK5 shares 97% and 99% aa identity with Human and Rat SLITRK5, respectively. The exact function of the LRRs is not known. They are assumed to mediate protein-protein-interactions (4). The cytoplasmic region contains two conserved, potential phosphorylation sites at Tyr833 and Tyr917, plus a TrkA-like tyrosine phosphorylation site (YLxxL) at Tyr945. SLITRK5 is expressed on embryonic neurons of the hippocampus (pyramidal layer) and thalamus (3). SLITRK5 was also shown to be expressed in hematopoietic stem cells, leukemias, and subsets of endothelial cells (5). Loss of SLITRK5 leads to manifestation of OCD-like behaviors in mice. SLITRK-5 was identified as an essential molecule at corticostriatal synapses (6). More recent studies have shown that SLITRK5 mediates BDNF-dependent biological functions through direct interaction with TrkB receptors. SLITRK5 primarily interacts with a trans-synaptic binding partner, protein tyrosine phosphatase delta (PTP delta), but upon BDNF stimulation it shifts to cis-interactions with TrkB (7).

References

  1. Aruga, J. et al. (2003) Gene 315:87.
  2. Nagase, T. et al. (2001) DNA Res. 8:179.
  3. Aruga, J. & K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.
  4. Enkhbayar, P. et al. (2003) Proteins 54:394.
  5. Milde T. (2007) Leukemia. 21:824.
  6. Shmelkov S.V. (2010) Nat. Med. 16:598.
  7. Song M. (2015) Dev. Cell. 33:690.

Long Name

SLIT and NTRK-like Family, Member 5

Alternate Names

KIAA0918, LRRC11

Entrez Gene IDs

26050 (Human); 75409 (Mouse); 306152 (Rat)

Gene Symbol

SLITRK5

UniProt

Q810B7

Additional SLITRK5 Products

Product Documents for Recombinant Mouse SLITRK5 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse SLITRK5 Protein, CF

For research use only

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