Recombinant Mouse SNED1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9335-SN

R&D Systems, part of Bio-Techne
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9335-SN-050
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Key Product Details

Source

CHO

Accession #

Q70E20

Conjugate

Unconjugated

Applications

Bioactivity

View all SNED1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse SNED1 protein
Arg23-Lys1403, with a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu28

Predicted Molecular Mass

150 kDa

SDS-PAGE

135-230 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of CCD-1070Sk human normal skin fibroblasts.
The ED50 for this effect is 0.1-0.4 μg/mL

Scientific Data Images for Recombinant Mouse SNED1 Protein, CF

Recombinant Mouse SNED1 Protein Bioactivity

Recombinant Mouse SNED1 Protein Bioactivity

Immobilized Recombinant Mouse SNED1 (Catalog # 9335-SN) supports the adhesion of CCD-1070Sk human normal skin fibroblasts The ED50 for this effect is 0.1-0.4 μg/mL.

Formulation, Preparation and Storage

9335-SN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SNED1

SNED1 (Sushi, nidogen and EGF-like domain-containing protein 1), also known as stromal nidogen extracellular matrix protein (Snep) and secreted protein SST-3, is an approximately 150 kDa protein (1). Mature mouse SNED1 contains 1379 aa that includes a Sushi/CCP (complement control protein) domain, an NIDO domain, 15 EGF-like domains, and 3 fibronectin type-III domains (1). Mouse SNED1 shares 84% and 96% aa sequence identity with human and rat SNED1, respectively. Alternative splicing generates an isoform lacking the Sushi/CCP domain, normally found in the center of the protein. SNED1 is mainly expressed in kidney stromal cells, but can be found in mesenchymal cells of other embryonic tissues, and within the nervous system (1,2). SNED1 has been found to have a role in tumor progression and metastasis. Studies show that SNED1 is up-regulated in highly metastatic mammary tumors while it is found to have relatively lower expression levels in poorly metastatic mammary tumors (3). SNED1 has also been shown to be up-regulated in pancreatic ductal adenocarcinoma cells which correlates with an increased chemoresistance (4). Additionally, SNED1 is found to be expressed specifically in stroma cells of the kidney and other organs in areas that undergo apoptosis during embryonic development making it important for kidney development (1).

References

  1. Leimeister, C. et al. (2004) Developmental Dynamics. 230:371.
  2. Ueno, H. et al. (2003) Nature Immunology. 4:457.
  3. Naba, A. et al. (2014) eLife. 3:e01308.
  4. Longati, P. et al. (2013) BMC Cancer. 13:95.

Long Name

Sushi, Nidogen and EGF-like Domain-containing Protein

Alternate Names

IRE-BP1, Snep

Entrez Gene IDs

25992 (Human); 208777 (Mouse); 316638 (Rat)

Gene Symbol

SNED1

UniProt

Q70E20

Additional SNED1 Products

Product Documents for Recombinant Mouse SNED1 Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Mouse SNED1 Protein, CF

For research use only

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