Recombinant Mouse ST3GAL5/GM3 Synthase Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8454-GT

R&D Systems, part of Bio-Techne
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8454-GT-050
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Key Product Details

Source

CHO

Accession #

O88829

Conjugate

Unconjugated

Applications

Enzyme Activity

View all ST3GAL5/GM3 Synthase Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse ST3GAL5/GM3 Synthase protein
Thr87-His414, with an N-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

38 kDa

SDS-PAGE

45-60 kDa, reducing conditions

Activity

Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to alpha-lactose.
The specific activity is >5 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8454-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: ST3GAL5/GM3 Synthase

Sialyltransferases add sialic acid to glycoproteins or glycosphingolipids and play important roles in many biological processes including immune recognition, pathogen infection, and cell adhesion (1). ST3GAL5 is a type II membrane protein localized in the trans-Golgi network that can transfer sialic acid to lactosylceramide (LacCer) to form GM3 (NeuAc alpha2-3Gal beta1-Glc beta1-Ceramide), the main ganglioside in human plasma (2). GM3 is known to participate in differentiation, proliferation and maintenance of fibroblast morphology (3). It also plays role in signal transduction, and integrin-mediated cell adhesion. Mutation of ST3GAL5 has been associated with Amish infantile epilepsy syndrome (4) and type 2 diabetes (5). Despite the ubiquitous distribution of ganglioside GM3 in human tissues, the expression of ST3GAL5 is found in a tissue-specific manner, with predominant expression in brain, skeletal muscle, and testis, and very low expression in liver (2). The enzymatic activity of recombinant mouse ST3GAL5 was determined using a phosphatase-coupled glycosyltransferase assay (6).

References

  1. Varki, A. (1999) Glycobiology 2:25.
  2. Ishii, A. et al (1998) J. Biol. Chem. 273:31652.
  3. Uemura, S. et al (2009) Mol. Biol. Cell 20:3088.
  4. Simpson, M. A. et al. (2004) Nat. Genet. 36:1225.
  5. Tagami, S. et al. (2002) J. Biol. Chem. 277:3085.
  6. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

ST3 beta-Galactoside alpha-2,3-Sialyltransferase 5

Alternate Names

3S-T, Ganglioside GM3 Synthase, SATI, Siat9, ST3GALV

Entrez Gene IDs

8869 (Human); 20454 (Mouse); 83505 (Rat)

Gene Symbol

ST3GAL5

UniProt

O88829

Additional ST3GAL5/GM3 Synthase Products

Product Documents for Recombinant Mouse ST3GAL5/GM3 Synthase Protein, CF

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Product Specific Notices for Recombinant Mouse ST3GAL5/GM3 Synthase Protein, CF

For research use only

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