Recombinant Mouse TACI/TNFRSF13B Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1041-TC
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse TACI/TNFRSF13B protein
| Mouse TACI (Phe5 - Thr129) Accession # Q9ET35 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
| N-terminus | C-terminus |
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Phe5
Predicted Molecular Mass
41 kDa (monomer)
SDS-PAGE
47 kDa, reducing conditions
Activity
Measured by its ability to inhibit BAFF-mediated proliferation of anti-IgM stimulated mouse B cells. Moore, P.A. et al. (1999) Science 285:260; Gross, J.A. et al. (2000) Nature 404:995; Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
The ED50 for this effect is 3-10 ng/mL in the presence of 2.5 ng/mL of rhBAFF.
The ED50 for this effect is 3-10 ng/mL in the presence of 2.5 ng/mL of rhBAFF.
Formulation, Preparation and Storage
1041-TC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TACI/TNFRSF13B
TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily and has been designated TNFRSF13B. TACI is a type III membrane protein with an extracellular N-terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine-rich domains. Within the TNFRSF, it shares the highest homology with B cell maturation factor (BCMA). TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Mouse TACI is a 249 amino acid (aa) protein consisting of a 129 aa extracellular domain, a 20 aa transmembrane domain, and a 100 aa intracellular domain. Mouse and human TACI share 54% aa identity.
References
- Xia, X.Z. et al. (2000) J. Exp. Med. 192:137.
- von Bulow, G.U. et al. (1997) Science 278:138.
- Gross, J.A. et al. (2000) Nature 404:995.
- Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
- Yan, M. et al. (2000) Nature Immunol. 1:37.
- Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.
Long Name
Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B
Alternate Names
CD267, CVID, TNFRSF13B
Gene Symbol
TNFRSF13B
UniProt
Additional TACI/TNFRSF13B Products
Product Documents for Recombinant Mouse TACI/TNFRSF13B Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse TACI/TNFRSF13B Fc Chimera Protein, CF
For research use only
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