Recombinant Mouse TAPBPR Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11254-TP

R&D Systems, part of Bio-Techne
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11254-TP-050
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Key Product Details

Source

NS0

Accession #

NP_663366.2

Conjugate

Unconjugated

Applications

Bioactivity

View all TAPBPR Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TAPBPR protein
Mouse TAPBPR
(Thr21-Gly412)
Accession # NP_663366.2		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr21

Predicted Molecular Mass

69 kDa

SDS-PAGE

75-90 kDa, under reducing conditions.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 0.200‑5.00 μg/mL.

Scientific Data Images for Recombinant Mouse TAPBPR Fc Chimera Protein, CF

Recombinant Mouse TAPBPR Fc Chimera Protein Bioactivity.

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion by human T cells. The ED50 for this effect is 0.200-5.00 μg/mL.

Recombinant Mouse TAPBPR Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse TAPBPR Fc Chimera Protein (Catalog # 11254-TP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-90 kDa and 150-180 kDa, respectively.

Formulation, Preparation and Storage

11254-TP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TAPBPR

TAP-binding protein-like (TAPBPL), also known as TAP binding protein-related (TAPBPR) and Tapasin-related protein (TAPASINR) is a transmembrane protein of the Immunoglobulin (Ig) superfamily (1, 2). TAPBPR was originally isolated as a homologue to TAPASIN but more recently was identified as a novel B7 family-related molecule since it shares sequence, structural, and functional similarities to B7 family members (3). Mature mouse TAPBPR consists of a lumenal domain containing an IgV and IgC domain, a transmembrane domain, and a cytoplasmic tail.  Within the lumenal domain, mature mouse TAPBPR shares 70% and 87% amino acid sequence identity with human and rat TAPBPR, respectively. Multiple alternatively spliced TAPBPR isoforms are known to exist with unique properties (4).TAPBPR is widely expressed and, similar to TAPASIN, functions as a both a chaperone protein and peptide editor of MHC class I, but in a peptide‑loading complex (PLC) independent manner (5, 6). TAPBPR decreases the rate at which MHC class I molecules mature through the secretory pathway, a role which could be important for peptide selection by MHC class I molecules (7). TAPBPR is also expressed on the surface of T cells and antigen-presenting cells (APCs) and plays an inhibitory role in the proliferation and activation of T cells (4). TAPBPR can be expressed on various cancer cells like leukemia and has the potential to be used in the treatment of autoimmune diseases and transplant rejection, as well as cancer (4).

References

  1. Hermann, C. et al. (2015) Tissue antigens 85(3):155.
  2. Teng, M. et al. (2002) European Journal of Immunology 32:1059.
  3. Lin, Y. et al. (2021). EMBO Mol Med. 13(5):13404.
  4. Porter, K.M. et al. (2014) Immunology 142:289.
  5. Margulies, D. et al. (2020) Current Opinion in Immunology 64:71.
  6. Boyle, L.H. et al. (2013) PNAS 110:3465.
  7. Hermann, C. et al. (2013) Journal of Immunology 191(11):5743.

Long Name

Tapasin Related Protein

Alternate Names

Tapasin-like, TAPBPL, TAPBPR

Entrez Gene IDs

55080 (Human); 213233 (Mouse); 297602 (Rat)

Gene Symbol

TAPBPL

UniProt

NP_663366.2

Additional TAPBPR Products

Product Documents for Recombinant Mouse TAPBPR Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse TAPBPR Fc Chimera Protein, CF

For research use only

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