Recombinant Mouse Thrombospondin-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7859-TH

R&D Systems, part of Bio-Techne
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7859-TH-050
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Key Product Details

Source

NS0

Accession #

NP_035710

Conjugate

Unconjugated

Applications

Bioactivity

View all Thrombospondin-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Thrombospondin-1 protein
Mouse Thrombospondin-1
(Asn19-Ser1171)
Accession # NP_035710		 GGGSGGGSGGGS 6-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Starts at Asn19

Predicted Molecular Mass

129 kDa

SDS-PAGE

130-155 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells.
The ED50 for this effect is 0.1-0.5 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 7859-TH in the following applications:

Formulation, Preparation and Storage

7859-TH
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombospondin-1

Thrombospondin‑1 (TSP‑1) is a 150‑180 kDa calcium‑sensitive protein that is secreted as a disulfide‑linked homotrimer. Within the Thrombospondin family, TSP‑2 is also homotrimeric, while TSP-3, -4, and -5/COMP are pentameric. TSP‑1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 contains an N‑terminal Laminin G‑like globular domain, an extended central region with one vWF‑C domain, 3 TSP type 1 repeats, 3 EGF‑like domains, and 8 TSP type‑3 repeats, and a globular C‑terminal lectin domain. Distinct regions of TSP‑1 have been associated with binding to particular ECM or cellular molecules (1, 2). Mature mouse TSP‑1 shares 95% and 99% amino acid sequence identity with human and rat TSP‑1, respectively. TSP‑1 counteracts the angiogenic, hypotensive, and anti‑thrombotic effects of nitric oxide (NO) (3). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells (EC), and destabilizes adhesive contacts between EC (4‑6). TSP‑1’s actions on vascular smooth muscle cells support arterial tone and blood pressure in the presence of vasodilatory stimulation (7). It is released from activated platelets and enhances platelet aggregation and adhesion (8). TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF‑beta and inducing release of the active cytokine from the latency associated peptide (LAP) (9, 10). TSP‑1 dampens adaptive immune responses by inducing the differentiation of regulatory T cells and inhibiting TCR signaling (11, 12). In the nervous system, it promotes excitatory synapse formation (13, 14) and supports the integration of neural progenitor cells into the olfactory bulb (15). In addition, TSP‑1 is released by apoptotic cells and promotes macrophage-mediated debris clearance (16).

References

  1. Murphy-Ullrich, J.E. and R.V. Iozzo (2012) Matrix Biol. 31:152.
  2. Laherty, C.D. et al. (1992) J. Biol. Chem. 267:3274.
  3. Roberts, D.D. et al. (2012) Matrix Biol. 31:162.
  4. Gupta, K. et al. (1999) Angiogenesis 3:147.
  5. Kaur, S. et al. (2010) J. Biol. Chem. 285:38923.
  6. Garg, P. et al. (2011) Am. J. Physiol. Lung Cell. Mol. Physiol. 301:L79.
  7. Isenberg, J.S. et al. (2009) Matrix Biol. 28:110.
  8. Isenberg, J.S. et al. (2008) Blood 111:613.
  9. Schultz-Cherry, S. et al. (1994) J. Biol. Chem. 269:26775.
  10. Sweetwyne, M.T. and J.E. Murphy-Ullrich (2012) Matrix Biol. 31:178.
  11. Grimbert, P. et al. (2006) J. Immunol. 177:3534.
  12. Kaur, S. et al. (2011) J. Biol. Chem. 286:14991.
  13. Xu, J. et al. (2010) Nat. Neurosci. 13:22.
  14. Eroglu, C. et al. (2009) Cell 139:380.
  15. Blake, S.M. et al. (2008) EMBO J. 27:3069.
  16. Moodley, Y. et al. (2003) Am. J. Pathol. 162:771.

Alternate Names

THBS1, Thrombospondin1, TSP-1

Entrez Gene IDs

7057 (Human); 21825 (Mouse)

Gene Symbol

THBS1

UniProt

NP_035710

Additional Thrombospondin-1 Products

Product Documents for Recombinant Mouse Thrombospondin-1 Protein, CF

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Product Specific Notices for Recombinant Mouse Thrombospondin-1 Protein, CF

For research use only

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