Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11220-TI

R&D Systems, part of Bio-Techne
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11220-TI-100
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Key Product Details

Source

NS0

Accession #

Q06806.3

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Tie-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Tie-1 protein
Mouse Tie-1
(Ser22-Ala749)
Accession # Q06806.3		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser22

Predicted Molecular Mass

106 kDa

SDS-PAGE

105-125 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Integrin alphaV beta3  (Catalog # 7889-AV) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse Tie-1 (1-749) Fc Chimera (Catalog # 11220-TI) binds with an ED50 of 2.00-20.0 μg/mL.

Scientific Data Images for Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein, CF

Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein Binding Activity.

When Recombinant Mouse Integrin alphaV beta3 (7889-AV) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse Tie‑1 (1-749) Fc Chimera (Catalog # 11220-TI) binds with an ED50 of 2.00-20.0 μg/mL.

Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein (Catalog # 11220-TI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-125 kDa and 210-250 kDa, respectively.

Formulation, Preparation and Storage

11220-TI
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tie-1

Tyrosine -protein kinase with immunoglobulin (Ig) like and epidermal growth factor (EGF) like domains 1 (Tie1), along with the closely related Tie2, is a transmembrane, vascular-specific receptor tyrosine kinase (RTKs) involved in angiogenesis, vascular development, and hematopoiesis (1,2). The Tie molecules are characterized by three Ig-like domains, three EGF-like domains, and three fibronectin type III-like repeats in the extracellular domain (ECD) and a split tyrosine kinase domain in the cytoplasmic region (3,4). The ECD of mouse Tie1 shares 89% amino acid sequence identity with human Tie1. Tie1 is expressed in endothelial and some hematopoietic progenitor cells and helps modulate angiopoietin signaling (5). While Tie1 is unable to directly bind any of the angiopoietins, it can modulate Tie2/angiopoietin signaling through Tie1/Tie2 heterodimer formation (6). Upregulation of Tie1 has been found in some leukemia and tumor related endothelial cells and inhibition of Tie1 has been shown to prevent metastasis and is being studied as a target for anti-angiogenesis treatments (7-9).

References

  1. Thomas, M. and Augustin, H. (2009) Angiogenesis 12:125.
  2. Fagiani, E. and Christofori, G. (2013) Cancer Letters 328:1.
  3. Seegar, T.C.M. et al. (2010). Mol. Cell. 37:643.
  4. Barton, W.A. et al. (2006). Nat Struc & Molec Biol. 13:524.
  5. Saharinen, P. et al. (2017) Nat Rev Drug Discov. 16:635.
  6. Yuan, H.T. et al. (2007) FASEB J. 21:3171.
  7. Yang, P. et al. (2015). J. Huazhong Univ. Sci. Technol. Med. Sci. 35:615-622.
  8. D'Amico, G. et al. (2014) J Clin Invest. 124:824.
  9. Khan, K.A. and Kerbel, R.S. (2020) EMBO molecular medicine 12:e12355.

Long Name

Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 1

Alternate Names

Tie1

Entrez Gene IDs

7075 (Human); 21846 (Mouse)

Gene Symbol

TIE1

UniProt

Q06806.3

Additional Tie-1 Products

Product Documents for Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein, CF

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