Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 11220-TI
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Tie-1 protein
Mouse Tie-1 (Ser22-Ala749) Accession # Q06806.3 |
IEGRMDP | Mouse IgG2A (Glu98-Lys330) |
N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser22
Predicted Molecular Mass
106 kDa
SDS-PAGE
105-125 kDa, under reducing conditions.
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Integrin alphaV beta3 (Catalog # 7889-AV) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse Tie-1 (1-749) Fc Chimera (Catalog # 11220-TI) binds with an ED50 of 2.00-20.0 μg/mL.
When Recombinant Mouse Integrin alphaV beta3 (Catalog # 7889-AV) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse Tie-1 (1-749) Fc Chimera (Catalog # 11220-TI) binds with an ED50 of 2.00-20.0 μg/mL.
Scientific Data Images for Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein, CF
Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein Binding Activity.
When Recombinant Mouse Integrin alphaV beta3 (7889-AV) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse Tie‑1 (1-749) Fc Chimera (Catalog # 11220-TI) binds with an ED50 of 2.00-20.0 μg/mL.Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein SDS-PAGE.
2 μg/lane of Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein (Catalog # 11220-TI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-125 kDa and 210-250 kDa, respectively.Formulation, Preparation and Storage
11220-TI
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Reconstitution | Reconstitute at 500 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Tie-1
References
- Thomas, M. and Augustin, H. (2009) Angiogenesis 12:125.
- Fagiani, E. and Christofori, G. (2013) Cancer Letters 328:1.
- Seegar, T.C.M. et al. (2010). Mol. Cell. 37:643.
- Barton, W.A. et al. (2006). Nat Struc & Molec Biol. 13:524.
- Saharinen, P. et al. (2017) Nat Rev Drug Discov. 16:635.
- Yuan, H.T. et al. (2007) FASEB J. 21:3171.
- Yang, P. et al. (2015). J. Huazhong Univ. Sci. Technol. Med. Sci. 35:615-622.
- D'Amico, G. et al. (2014) J Clin Invest. 124:824.
- Khan, K.A. and Kerbel, R.S. (2020) EMBO molecular medicine 12:e12355.
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 1
Alternate Names
Tie1
Gene Symbol
TIE1
UniProt
Additional Tie-1 Products
Product Documents for Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein, CF
For research use only
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