Recombinant Mouse TIM-2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1885-TM

R&D Systems, part of Bio-Techne
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1885-TM-050
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Key Product Details

Source

NS0

Accession #

NP_599010

Conjugate

Unconjugated

Applications

Bioactivity

View all TIM-2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TIM-2 protein
Met1-Asn229, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His22

Predicted Molecular Mass

23.5 kDa

SDS-PAGE

40-60 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

Immobilized Recombinant Mouse TIM-2 (0.5 μg/mL, 100 μL/well) can bind H-ferritin with an apparent KD <3 nM.

Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

1885-TM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-2

T cell immunoglobulin and mucin domain‑2 (TIM‑2) is an approximately 50 kDa member of the TIM family of immune regulatory proteins. TIMs are type I transmembrane proteins with one Ig‑like V domain and a Ser/Thr‑rich mucin domain in their extracellular regions (1). Mature mouse TIM‑2 consists of a 210 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 53 aa cytoplasmic domain (2). Rat TIM‑2, by contrast, possesses the same structural architecture as mouse but contains an additional 46 aa within the mucin domain. Over comparable regions of the ECD, mouse and rat TIM‑2 share 74% aa sequence identity. The isolated ECD forms dimers and tetramers in solution, and these structures suggest the possibility of TIM‑2/TIM‑2 cis interactions (3). TIM‑2 is expressed on Th2 cells, B cells, oligodendrocytes, hepatocytes, hepatic stellate cells, and epithelial cells lining the bile duct and renal tubules (4 ‑ 9). It is upregulated on Th2 cells during cellular activation and functions as a negative regulator of Th2 cell expansion and immune responses (8 ‑ 11). TIM‑2 binds to ligands expressed on activated B cells, macrophages, dendritic cells, and T cells (8, 12). Its ligation by Semaphorin 4A induces tyrosine phosphorylation in the cytoplasmic domain of TIM‑2 (12). TIM‑2 binding to ligand(s) on hepatocytes inhibits hepatocyte differentiation and proliferation (7). TIM‑2 additionally plays a role in iron homeostasis by serving as an endocytic receptor for the heavy chain of Ferritin (H‑Ferritin) (4, 6).

References

  1. Rodriguez-Manzanet, R. et al. (2009) Immunol. Rev. 229:259.
  2. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  3. Santiago, C. et al. (2007) Immunity 26:299.
  4. Todorich, B. et al. (2008) J. Neurochem. 107:1495.
  5. Ruddell, R.G. et al. (2009) Hepatology 49:887.
  6. Chen, T.T. et al. (2005) J. Exp. Med. 202:955.
  7. Watanabe, N. et al. (2007) Hepatology 45:1240.
  8. Chakravarti, S. et al. (2005) J. Exp. Med. 202:437.
  9. Rennert, P.D. et al. (2006) J. Immunol. 177:4311.
  10. Fukushima, A. et al. (2007) Immunol. Lett. 110:133.
  11. Knickelbein, J.E. et al. (2006) J. Immunol. 177:4966.
  12. Kumanogoh, A. et al. (2002) Nature 419:629.

Long Name

T Cell Immunoglobulin Mucin-2

Alternate Names

TIM2

Entrez Gene IDs

171284 (Mouse)

Gene Symbol

TIMD2

UniProt

NP_599010

Additional TIM-2 Products

Product Documents for Recombinant Mouse TIM-2 Protein, CF

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Product Specific Notices for Recombinant Mouse TIM-2 Protein, CF

For research use only

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