Recombinant Mouse Transglutaminase 2/TGM2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 5418-TG
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Transglutaminase 2/TGM2 protein
Ala2-Ala686, with an N-terminal Met and 6-His tag
Ala2-Ala686, with an N-terminal Met and 6-His tag
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
78 kDa
SDS-PAGE
78 kDa, reducing conditions
Activity
Measured by its ability to form CBZ-Gln-Gly-Hydroxamate from CBZ-Gln-Gly and Hydroxylamine.
The specific activity is >1,300 pmol/min/μg, as measured under the described conditions.
The specific activity is >1,300 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
5418-TG
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Transglutaminase 2/TGM2
Transglutaminase 2 (TG2), encoded by the Tgm2 gene, is also known as tissue Transglutaminase (tTG), Transglutaminase C (TGC), and protein-glutamine-gamma ‑glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1‑3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6, 7).
References
- Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
- Chen, J.S.K. and Mehta K. (1999) Internat. J. Biochem. Cell Biol. 31:817.
- Griffin, M. et al. (2002) Biochem. J. 368:377.
- Lai, T.S. et al. (1998) J. Biol. Chem. 273:1776.
- Mishra, S. et al. (2007) J. Biol. Chem. 282:18108.
- Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.
- Lesort, M. et al. (2000) Prog. Neurobiol. 61:439.
Alternate Names
TGC, TGM2, Transglutaminase 2, tTG
Gene Symbol
TGM2
UniProt
Additional Transglutaminase 2/TGM2 Products
Product Documents for Recombinant Mouse Transglutaminase 2/TGM2 Protein, CF
Product Specific Notices for Recombinant Mouse Transglutaminase 2/TGM2 Protein, CF
For research use only
Loading...
Loading...
Loading...