Recombinant Mouse TSH alpha/beta Heterodimer Protein
R&D Systems, part of Bio-Techne | Catalog # 8885-TH
Key Product Details
Source
Conjugate
Applications
Product Specifications
Source
Mouse TSH beta (Phe21-Val138) Accession # P12656 |
(GGGS)3 |
Mouse TSH alpha (Leu25-Ser120) Accession # P01216 |
N-terminus | C-terminus |
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is 0.015-0.15 ng/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 8885-TH
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 8885-TH/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TSH alpha/beta Heterodimer
half-lives exist due to differences in the post-translational glycosylation and sialylation modifications of its subunits (1, 4). TSH is produced and secreted by the anterior pituitary gland. Its secretion is controlled by Thyrotropin-Releasing Hormone (TRH) from the hypothalamus. TSH travels to the thyroid gland and binds to the TSH Receptor to stimulate production of Thyroxine (T4), which is converted in peripheral tissues to Triiodothyronine (T3), a more biologically potent form of the thyroid hormone (1, 4, 5). TSH secretion is also controlled by plasma T3 and T4 via a negative feedback mechanism and by the neurotransmitters dopamine and Somatostatin (4, 5). Serum TSH levels are often assessed to evaluate thyroid function (4, 5). TSH has also been shown to be produced by bone marrow and is believed to have a direct effect on skeletal homeostasis by promoting bone strength and quality (6-9). Additionally, serum TSH levels have been shown to be decreased in the elderly (10).
References
- Szkudlinshi, M.W. et al. (2002) Physiol. Rev. 82:473.
- Farid, N.R. and M.W. Szkudlinski (2004) Endocrinology 145:4048.
- Cahoreau, C. et al. (2015) Front. Endocrinol. (Lausanne) 6:26.
- Roelfsema, F. and J.D. Veldhuis (2013) Endocr. Rev. 34:619.
- Winter, W.E. and M.R. Signorino (2001) Ann. Clin. Lab. Sci. 31:221.
- Klein, J.R. and H.C. Wang (2004) J. Exp. Biol. 207:55.
- Abe, E. et al. (2003) Cell 115:151.
- Sun, L. et al. (2006) Ann. N.Y. Acad. Sci. 1068:309.
- Sendak, R.A. et al. (2007) Int. Orthop. 31:753.
- Peeters, R.P. (2008) Hormones 7:28.
Long Name
Alternate Names
Additional TSH alpha/beta Heterodimer Products
Product Documents for Recombinant Mouse TSH alpha/beta Heterodimer Protein
Product Specific Notices for Recombinant Mouse TSH alpha/beta Heterodimer Protein
For research use only