Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8948-VN

R&D Systems, part of Bio-Techne
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8948-VN-050
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Key Product Details

Source

NS0

Accession #

Q9CZT5

Conjugate

Unconjugated

Applications

Bioactivity

View all Vasorin/SLIT-like 2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Vasorin/SLIT-like 2 protein
Cys25-Pro576, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys25

Predicted Molecular Mass

61 kDa

SDS-PAGE

72-97 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse TGF- beta2 (Catalog # 7346-B2) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Mouse Vasorin/SLIT-like 2 that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL

Formulation, Preparation and Storage

8948-VN
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, Ethylene Glycol and Trehalose.
Reconstitution
Reconstitute at 250 μg/mL in water.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage

Background: Vasorin/SLIT-like 2

Vasorin, also known as SLIT-like 2, is an approximately 110 kDa transmembrane protein that is expressed on vascular smooth muscle cells and in the developing skeletal system (1, 2). It is down-regulated following vascular injury (1). A 95 kDa soluble form of Vasorin can be shed from the cell surface by ADAM17 mediated cleavage (3). The shed soluble form of Vasorin binds to TGF-beta 1, 2, and 3 and prevents TGF-beta induced effects including intimal formation and epithelial mesenchymal transition (1, 3). Soluble Vasorin is elevated in the serum of hepatic carcinoma patients relative to normal or non-cancerous hepatic disease (4). It promotes tumor cell proliferation, migration, and survival (4). Mature mouse Vasorin consists of a 552 amino acid (aa) extracellular domain (ECD) with 10 leucine rich repeats (LRR) flanked by one LRRNT and one LRRCT domain, one EGF-like domain, and one Fibronectin type-III domain, a 21 aa transmembrane segment, and a 76 aa cytoplasmic domain (1). Within the ECD, mouse Vasorin shares 85% and 96% aa sequence identity with human and rat Vasorin, respectively.

References

  1. Ikeda, Y. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10732.
  2. Krautzberger, A.M. et al. (2012) Gene Expr. Patterns 12:167.
  3. Malapeira, J. et al. (2011) Oncogene 30:1912.
  4. Li, S. et al. (2015) Oncotarget 6:10045.

Alternate Names

Atia, SLIT like 2, Slitl2, VASN

Entrez Gene IDs

114990 (Human); 246154 (Mouse)

Gene Symbol

VASN

UniProt

Q9CZT5

Additional Vasorin/SLIT-like 2 Products

Product Documents for Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF

For research use only

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