Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 643-VM
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse VCAM-1/CD106 protein
| Met | Mouse VCAM-1 (Phe25-Glu698) Accession # CAA47989 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | |||
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
101.9 kDa (monomer)
SDS-PAGE
120 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.
When 5 x 104 cells/well are added to mouse VCAM-1 coated plates (10 µg/mL with 100 µL/well), >85% will adhere after 1 hour incubation at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
When 5 x 104 cells/well are added to mouse VCAM-1 coated plates (10 µg/mL with 100 µL/well), >85% will adhere after 1 hour incubation at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
643-VM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 1 mg/mL in sterile, deionized water.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: VCAM-1/CD106
During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha4 beta7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.
ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.
Long Name
Vascular Cell Adhesion Molecule 1
Alternate Names
CD106, VCAM1
Gene Symbol
VCAM1
UniProt
Additional VCAM-1/CD106 Products
Product Documents for Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF
For research use only
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