Recombinant Mouse VISTA/B7-H5/PD-1H Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7005-B7

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

Q9D659

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all VISTA/B7-H5/PD-1H Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse VISTA/B7-H5/PD-1H protein
Mouse VISTA/B7-H5/PD-1H
(Phe33-Ala191)
Accession # NP_001153044		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe33

Predicted Molecular Mass

45 kDa (monomer)

SDS-PAGE

60-70 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes.
The ED50 for this effect is 0.5-3 μg/mL.

Scientific Data Images for Recombinant Mouse VISTA/B7-H5/PD-1H Fc Chimera Protein, CF

Recombinant Mouse VISTA/B7-H5/PD-1H Fc Chimera Protein Bioactivity

Recombinant Mouse VISTA/B7-H5/PD-1H Fc Chimera Protein Bioactivity

Recombinant Mouse VISTA/B7-H5/PD-1H Fc Chimera (Catalog # 7005-B7) inhibits anti-CD3 antibody-induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-3 μg/mL.

Formulation, Preparation and Storage

7005-B7
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VISTA/B7-H5/PD-1H

Platelet receptor Gi24, also known as Dies1, VISTA, SISP1 and B7-H5, is a 55-65 kDa transmembrane glycoprotein with homology to B7-like immune co-stimulatory molecules (1, 2). It is distinct from HHLA2 which is also known as B7-H5 (3, 4). Mature mouse Gi24 contains a 159 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane segment, and a 97 aa cytoplasmic domain. Within the ECD, mouse Gi24 shares 70% and 78% aa sequence identity with human and rat Gi24, respectively (5). The 30 kDa ECD can be shed by MT1-MMP, with a 25-30 kDa fragment remaining in the membrane (6). Gi24 promotes both MT1-MMP expression and the MT1-MMP mediated activation of MMP-2 (6). Gi24 supports the differentiation of embryonic stem cells (ESC) and enhances BMP-4 induced signaling in ESC, but it is also down-regulated following BMP-4 exposure (7, 8). It binds to BMP-4 directly and also associates with the type I BMP receptor Activin RIB/ALK-4 (7, 8). Gi24 is expressed on the surface of naïve CD4+ T cells and regulatory T cells (5). It is up-regulated in vivo on activated monocytes and dendritic cells (8). Gi24 inhibits CD4+ and CD8+ T cell proliferation and their production of IL-2 and IFN-gamma (5). Its expression on tumor cells attenuates the anti-tumor immune response and enables more rapid tumor progression (5). In contrast, Gi24 limits disease progression in the autoimmune disease model EAE (5). Gi24 is additionally expressed on mature adipocytes, particularly in fasting mice (9).

References

  1. Jung, K. and I. Choi (2013) Immune Netw. 13:184.
  2. Wilcox, R.A. et al. (2012) Eur. J. Haematol. 88:465.
  3. Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
  4. Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.
  5. Wang, L. et al. (2011) J. Exp. Med. 208:577.
  6. Sakr, M.A. et al. (2010) Cancer Sci. 101:2368.
  7. Aloia, L. et al. (2010) J. Biol. Chem. 285:7776.
  8. Parisi, S. et al. (2012) FASEB J. 26:3957.
  9. Ren, G. et al. (2013) PLoS ONE 8:e65531.

Alternate Names

4632428N05Rik, B7-H5, C10orf54, Dies1, Gi24, PD-1H, PD1H, SISP1, VSIR

Entrez Gene IDs

64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)

Gene Symbol

VSIR

UniProt

Q9D659 (Mouse)

Additional VISTA/B7-H5/PD-1H Products

Product Documents for Recombinant Mouse VISTA/B7-H5/PD-1H Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse VISTA/B7-H5/PD-1H Fc Chimera Protein, CF

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