Recombinant Mouse VSIG10 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9604-VS

R&D Systems, part of Bio-Techne
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9604-VS-050
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Key Product Details

Source

NS0

Accession #

D3YX43

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all VSIG10 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse VSIG10 protein
Mouse VSIG10
(Leu25-Asn426)
Accession # D3YX43		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu25

Predicted Molecular Mass

71 kDa

SDS-PAGE

97-118 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-17 secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 0.15-0.9 μg/mL.

Scientific Data Images for Recombinant Mouse VSIG10 Fc Chimera Protein, CF

Recombinant Mouse VSIG10 Fc Chimera Protein Bioactivity

Recombinant Mouse VSIG10 Fc Chimera Protein Bioactivity

Recombinant Mouse VSIG10 Fc Chimera (Catalog # 9604-VS) inhibits anti-CD3 antibody induced IL-17 secretion in human PBMC. The ED50 for this effect is 0.15-0.9 μg/mL.

Formulation, Preparation and Storage

9604-VS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG10

VSIG10 (V-set and immunoglobulin domain-containing protein 10) is a single-pass type I membrane protein of the Ig superfamily. Mouse VSIG10 cDNA encodes a 558 amino acid (aa) precursor that contains a 20 aa signal sequence, a 406 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 111 aa cytoplasmic domain (1). The ECD of mouse VSIG10 contains four Ig-like C2-type domains and shares 63% and 84% aa identity with human and rat VSIG10, respectively. The specific biological function of VSIG10L has yet to be elucidated, but there is evidence to suggest the molecule is involved in cell-cell adhesion. Ig‑like C2-type domains are similar to antibody constant domains and can be found in T cell adhesion molecules, such as Cluster of Differentiation 2 (CD2) along with vascular and intercellular cell adhesion molecules (VCAM and ICAM, respectively) (2). VSIG10 is structurally related to the B7 family of immune regulatory proteins. Our studies at R&D Systems show that VSIG10 inhibits T cell activation, including IL-17 and Interferon gamma production.

References

  1. SwissProt Accession # D3YX43.
  2. Yang H. and E. L. Reinherz (2001) J. Biol. Chem. 276:18775.

Long Name

V-Set and Immunoglobulin Domain Containing 10

Alternate Names

FLJ20674, V-set and immunoglobulin domain containing 10, V-set and immunoglobulin domain-containing protein 10

Entrez Gene IDs

54621 (Human); 231668 (Mouse); 304529 (Rat)

Gene Symbol

VSIG10

UniProt

D3YX43

Additional VSIG10 Products

Product Documents for Recombinant Mouse VSIG10 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse VSIG10 Fc Chimera Protein, CF

For research use only

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