Recombinant Mouse VSIG3 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9367-VS

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

P0C673

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all VSIG3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse VSIG3 protein
Mouse VSIG3
(Leu23-Gly245)
Accession # P0C673		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu23

Predicted Molecular Mass

51 kDa

SDS-PAGE

60-68 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-17 secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 1-5 μg/mL

Scientific Data Images for Recombinant Mouse VSIG3 Fc Chimera Protein, CF

Recombinant Mouse VSIG3 Fc Chimera Protein Bioactivity

Recombinant Mouse VSIG3 Fc Chimera Protein Bioactivity

Recombinant Mouse VSIG3 Fc Chimera (Catalog # 9367-VS) inhibits anti-CD3 antibody induced IL-17 secretion in human PBMC. The ED50 for this effect is 1-5 μg/mL.

Formulation, Preparation and Storage

9367-VS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG3

VSIG3 (V-­set and immunoglobulin domain containing 3) also known as IgSF11 (Immunoglobulin superfamily member 11), is an approximately 50 kDa type I transmembrane adhesion protein of the Ig superfamily. Mature mouse VSIG3 consists of a 218 amino acid (aa) extracellular domain (ECD) containing one V-type Ig-like domain and one Ig-like C2-type domain, a 21 aa transmembrane domain, and a 167 aa cytoplasmic domain. Within the ECD, mouse VSIG3 shares 95% and 98% aa identity with human and rat VSIG3, respectively. VSIG3 is expressed on epithelial and endothelial cells, neurons and glial cells, and platelets (1). It localizes to epithelial tight junctions and mediates homophilic in trans cell adhesion (1-3). VSIG3 also localizes to neuronal postsynaptic densities where it recruits the GluA1 and GluA2 subunits of AMPA receptors and supports excitatory synaptic transmission (4). In zebrafish, VSIG3 is expressed in melanophores and their precursors and plays a role in the development and patterning of pigment cells (5). VSIG-3 expression is elevated in colorectal cancers and hepatocellular carcinomas as well as
intestinal-type gastric cancers. Suppression of VSIG-3 by siRNA retarded the growth of gastric cancer cells, suggesting that VSIG-3 is a good candidate for gastric cancer immunotherapy (6).

References

  1. Raschperger, E. et al. (2004) J. Biol. Chem. 279:796.
  2. Katoh, M. and M. Katoh (2003) Int. J. Oncol. 23:525.
  3. Harada, H. et al. (2005) J. Cell. Physiol. 204:919.
  4. Jang, S. et al. (2016) Nat. Neurosci. 19:84.
  5. Eom, D.S. et al. (2012) PLoS Genet. 8:e1002899.
  6. Watanabe T, et al. (2005) Cancer Sci. 96:498.

Long Name

V-Set and Immunoglobulin Domain Containing 3

Alternate Names

BTIGSF, CXADRL1, IGSF11

Entrez Gene IDs

152404 (Human); 207683 (Mouse); 303926 (Rat); 102146257 (Cynomolgus Monkey)

Gene Symbol

IGSF11

UniProt

P0C673

Additional VSIG3 Products

Product Documents for Recombinant Mouse VSIG3 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse VSIG3 Fc Chimera Protein, CF

For research use only

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