Recombinant Mouse Wnt-3/sFRP-1 Complex Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11200-WN

R&D Systems, part of Bio-Techne
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Key Product Details

Source

CHO

Accession #

P17553.1, Q8C4U3.3

Structure / Form

Non-covalent complex

Conjugate

Unconjugated

Applications

Bioactivity

View all Wnt-3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-3 protein
Mouse Wnt-3
(Gly22-Lys355)
Accession # P17553.1
Mouse sFRP-1
(Ser32-Lys314)
Accession # Q8C4U3.3
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly22 (Wnt-3) & Ser32 (sFRP-1)

Predicted Molecular Mass

37 kDa (Wnt-3) & 33 kDa (sFRP-1)

SDS-PAGE

36-45 kDa, under reducing conditions.

Activity

Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells.
The ED50 for this effect is 7.00-63.0 ng/mL.

Scientific Data Images for Recombinant Mouse Wnt-3/sFRP-1 Complex Protein, CF

Recombinant Mouse Wnt-3/sFRP-1 Complex Bioactivity.

Recombinant Mouse Wnt-3/sFRP-1 Complex (Catalog # 11200-WN) activates Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 7.00-63.0 ng/mL.

Formulation, Preparation and Storage

11200-WN
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-3

Wnt-3 is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins that are important for normal developmental processes (1). Mouse Wnt-3 shares 99% and 100% amino acid (aa) identity with human and rat Wnt-3, respectively. It also shares 87% aa identity with mouse Wnt3a. Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta-catenin levels (4). As intracellular beta-catenin levels rise, it binds to TCF/LEF transcription factors, leading to expression of Wnt target genes (5). Wnts are known to bind to sFRPs, a family of secreted molecules that contain an N-terminal cysteine-rich domain (CRD) highly similar to the CRDs of the Frizzled family receptors (6,7). More than one molecule of sFRP is suggested to participate in the interaction with each Wnt molecule (7). Both sFRP-1 and sFRP-2 can inhibit Wnt-3a activity while sFRP-3 cannot (8). R&D Systems produces biologically active mouse Wnt-3 protein in a complex with mouse sFRP-1, and this Wnt-3/sFRP-1 complex is stable in the physiological buffer without detergent.

References

  1. Willert, K. and R. Nusse (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
  2. Van Ooyen, A. et al. (1985) EMBO J. 4:2905.
  3. Burrus, L.W. and A.P. McMahon (1995) Exp. Cell Res. 220:363.
  4. MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
  5. Korinek, V. et al. (1997) Science 275:1784.
  6. Dennis, S. et al. (1999) J. Cell Sci. 112:3815.
  7. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.
  8. Galli, L.M. et al. (2006) Dev. Dyn. 235:681.

Long Name

Wingless-type MMTV Integration Site Family, Member 3

Alternate Names

INT4, TETAMS, WNT3

Entrez Gene IDs

7473 (Human); 22415 (Mouse)

Gene Symbol

WNT3

UniProt

P17553.1, Q8C4U3.3

Additional Wnt-3 Products

Product Documents for Recombinant Mouse Wnt-3/sFRP-1 Complex Protein, CF

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Product Specific Notices for Recombinant Mouse Wnt-3/sFRP-1 Complex Protein, CF

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