Recombinant Porcine IFN-alpha (Mammalian) Protein

Name: Recombinant Porcine IFN-alpha (Mammalian) Protein
Brand: R&D Systems
SKU: 17105-1

R&D Systems, part of Bio-Techne | Catalog # 17105-1

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Technical Data
Citations & Reviews

Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Accession #

NM_214393.1

Conjugate

Unconjugated

Applications

Bioactivity

View all IFN-alpha Proteins and Enzymes »

Product Specifications

Source

cDNA obtained from porcine leukocyte mRNA expressed in mammalian cells.
Accession # NM_214393.1

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

19.1 kDa

Activity

Interferon was titrated with the use of the cytopathic effect inhibition assay as listed

Bovine (MDBK/VSV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37 (2):755]. The EC₅₀ for interferon in this assay is ~5 U/ml. Lot Activity was derived from multiple determinations in the above assay.

The units are determined by use of a Human IFN Alpha A (Hu IFN-alpha A [2a]) laboratory standard calibrated to the international reference standard for Human Interferon Alpha A (Hu IFN-alpha A [2a]) provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol Methods. 257(1-2):17-33]. Please note that IFN assays vary between labs and assay systems [Meager, et al (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].

Formulation, Preparation and Storage

17105-1

Formulation	Phosphate buffered saline with 0.1% bovine serum albumin (BSA)
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IFN-alpha

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Long Name

Interferon alpha

Alternate Names

IFNalpha, LeIF D

Entrez Gene IDs

3440 (Human)

Gene Symbol

IFNA2

UniProt