Recombinant Porcine IL-8/CXCL8 Protein

R&D Systems, part of Bio-Techne | Catalog # 535-IN

R&D Systems, part of Bio-Techne
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Carrier Free
535-IN-025/CF
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With Carrier
535-IN-025
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Key Product Details

Source

E. coli

Accession #

CAA43461

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-8/CXCL8 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived porcine IL-8/CXCL8 protein
Ala26-Gln104

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala26

Predicted Molecular Mass

9.4 kDa

Activity

Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474.
The ED50 for this effect is 0.4-0.6 µg/mL.

Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CXCR2.
The ED50 for this effect is 1-5 ng/mL.

Reviewed Applications

Read 2 reviews rated 5 using 535-IN in the following applications:

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 535-IN
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 535-IN/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-8/CXCL8

Interleukin-8 (IL-8), also known as CXCL8, GCP-1, and NAP-1, is a widely expressed proinflammatory member of the CXC family of chemokines. This 8-9 kDa chemokine circulates as a monomer, homodimer, and heterodimer with CXCL4/PF4 (1, 2). Its oligomerization is modulated by interactions with matrix and cell surface glycosaminoglycans (GAGs) (3). Mature porcine CXCL8 shares 82%, 78%, and 65% amino acid (aa) sequence identiity with canine, feline, and human CXCL8 (4). There is no CXCL8 gene counterpart in rodent. N-terminal truncation of CXCL8 by multiple proteases generates a range of shorter forms (5). The bioactivity of CXCL8 is regulated by these truncations and also by CXCL8 citrullination (6). CXCL8 effects are mediated through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines (1). These receptors associate into functional homodimers and heterodimers with each other (7). Through both CXCR1 and CXCR2, CXCL8 promotes neutrophil adhesion to the vascular endothelium and migration to sites of inflammation (8). It triggers the antimicrobial activation of neutrophils through CXCR1 (9). CXCL8 also binds to Serpin A1/alpha-1 Antitrypsin, and this prevents CXCL8 interaction with CXCR1 (10). CXCL8 is up-regulated in atherosclerotic lesions and other cardiac pathologies where it exacerbates inflammatory tissue damage (11). In addition, it induces VEGF expression, vascular endothelial cell proliferation, angiogenesis, and tumor cell invasiveness (12-15).

References

  1. Lazennec, G. and A. Richmond (2010) Trends Mol. Med. 16:133.
  2. Nesmelova, I.V. et al. (2005) J. Biol. Chem. 280:4948.
  3. Pichert, A. et al. (2012) Biomatter 2:142.
  4. Goodman, R.B. et al. (1992) Biochemistry 31:10483.
  5. Mortier, A. et al. (2008) Pharmacol. Ther. 120:197.
  6. Proost, P. et al. (2008) J. Exp. Med. 205:2085.
  7. Munoz, L.M. et al. (2009) J. Immunol. 183:7337.
  8. Gerszten, R.E. et al. (1999) Nature 398:718.
  9. Jones, S.A. et al. (1996) Proc. Natl. Acad. Sci. USA 93:6682.
  10. Bergin, D.A. et al. (2010) J. Clin. Invest. 120:4236.
  11. Apostolakis, S. et al. (2009) Cardiovasc. Res. 84:353.
  12. Martin, D. et al. (2009) J. Biol. Chem. 284:6038.
  13. Li, A. et al. (2005) Angiogenesis 8:63.
  14. Waugh, D.J. and C. Wilson (2008) Clin. Cancer Res. 14:6735.
  15. Fernando, R.I. et al. (2011) Cancer Res. 71:5296.

Long Name

Interleukin 8

Alternate Names

CXCL8, GCP1, IL8, LAI, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, NCF, TCF, TSG1

Entrez Gene IDs

3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)

Gene Symbol

CXCL8

UniProt

CAA43461

Additional IL-8/CXCL8 Products

Product Documents for Recombinant Porcine IL-8/CXCL8 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 535-IN (with carrier)
Download SDS 535-IN/CF (carrier free)

Product Specific Notices for Recombinant Porcine IL-8/CXCL8 Protein

For research use only

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