Recombinant Rat BMP-6 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10420-BM

R&D Systems, part of Bio-Techne
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10420-BM-020
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Key Product Details

Source

CHO

Accession #

Q04906.2

Structure / Form

Covalent dimer

Conjugate

Unconjugated

Applications

Bioactivity

View all BMP-6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived rat BMP-6 protein
Ser368-His506

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser368

Predicted Molecular Mass

16 kDa

SDS-PAGE

16-22 kDa, under reducing conditions

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.
The ED50 for this effect is 0.15-0.9 μg/mL.

Scientific Data Images for Recombinant Rat BMP-6 Protein, CF

Recombinant Rat BMP-6 Protein Bioactivity

Recombinant Rat BMP-6 Protein Bioactivity

Recombinant Rat BMP-6 (Catalog # 10420-BM) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 0.15-0.9 μg/mL.
Recombinant Rat BMP-6 Protein SDS-PAGE

Recombinant Rat BMP-6 Protein SDS-PAGE

2 μg/lane of Recombinant Rat BMP-6 (Catalog # 10420-BM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 16-22 kDa and 32-44 kDa, respectively.

Formulation, Preparation and Storage

10420-BM
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 200 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-6

Bone Morphogenetic Protein 6 (BMP-6), also known as Vgr-1, is a member of the BMP subfamily of TGF-beta superfamily proteins. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis (1). BMP-6 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release a smaller, C-terminal mature protein. Biologically active BMP-6 consists of a disulfide-linked homodimer of the mature protein, with each monomer containing a characteristic cystine knot motif (2). Mature rat BMP-6 shares 98% and 96% amino acid sequence identity with human and mouse BMP-6, respectively. Cellular responses to BMP-6 are mediated by hetero-oligomeric complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) serine/threonine kinase receptors (3-5). BMP-6 induces the expression of Noggin and is subsequently antagonized by Noggin (6). BMP-6 induces a wide range of cellular responses. It promotes osteoblast differentiation from mesenchymal stem cells (7), chondrocyte maturation (8), Ang II-induced aldosterone production in the adrenal cortex (4), hormone production and responsiveness in ovarian granulosa cells (9), iNOS and TNF-alpha production in macrophages (5), the cell death of B cells (10), and neurite outgrowth (11). BMP-6 expression is induced in astrocytes surrounding sites of brain injury where it functions as a neuroprotectant (11, 12). It enhances tumor progression by promoting local angiogenesis and differentiation of immune tolerizing M2 macrophages (13-15). Through interactions with the BMP co-receptor RGM-C/Hemojuvelin, BMP-6 plays an important role in iron homeostasis by promoting Hepcidin expression and preventing serum iron overload (16). Heterodimers of BMP-2 and BMP-6 show increased potency at inducing osteoblastic calcium deposition, chondrogenesis, and in vivo bone formation compared to either BMP-2 or BMP-6 homodimers (3).

References

  1. Bragdon, B. et al. (2010) Cell Signal. 23:609.
  2. Allendorph GP et al. (2007) Biochemistry. 46:12238.
  3. Israel, D.I. et al. (1996) Growth Factors 13:291.
  4. Inagaki, K. et al. (2006) Endocrinology 147:2681.
  5. Hong, J.H. et al. (2008) Immunology 128:e442.
  6. Haudenschild, D.R. et al. (2004) Cancer Res. 64:8276.
  7. Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
  8. Grimsrud, C.D. et al. (1999) J. Bone Miner. Res. 14:475.
  9. Shi, J. et al. (2009) Fertil. Steril. 92:1794.
  10. Kersten, C. et al. (2005) BMC Immunol. 6:9.
  11. Yabe, T. et al. (2002) J. Neurosci. Res. 68:161.
  12. Zhang, Z. et al. (2006) Neuroscience 138:47.
  13. Dai, J. et al. (2005) Cancer Res. 65:8274.
  14. Kwon, S.J. et al. (2014) Prostate 74:121.
  15. Lee, J.-H. et al. (2013) Cancer Res. 73:3604.
  16. Andriopoulos, B. Jr. et al. (2009) Nat. Genet. 41:482.

Long Name

Bone Morphogenetic Protein 6

Alternate Names

BMP6, Vgr-1

Entrez Gene IDs

654 (Human); 12161 (Mouse); 25644 (Rat)

Gene Symbol

BMP6

UniProt

Q04906.2

Additional BMP-6 Products

Product Documents for Recombinant Rat BMP-6 Protein, CF

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Product Specific Notices for Recombinant Rat BMP-6 Protein, CF

For research use only

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