Recombinant Rat CD155/PVR His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9764-CD

R&D Systems, part of Bio-Techne
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9764-CD-050
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Key Product Details

Source

HEK293

Accession #

NP_058772

Conjugate

Unconjugated

Applications

Bioactivity

View all CD155/PVR Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived rat CD155/PVR protein
Glu34-Leu351, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu34

Predicted Molecular Mass

35 kDa

SDS-PAGE

62-71 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Rat CD155/PVR is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD96 (Catalog # 5690-CD) that produces 50% optimal binding response is 1.5-7.5 ng/mL.

Scientific Data Images for Recombinant Rat CD155/PVR His-tag Protein, CF

Recombinant Rat CD155/PVR His-tag Protein Bioactivity

Recombinant Rat CD155/PVR His-tag Protein Bioactivity

When Recombinant Rat CD155/PVR (Catalog # 9764-CD) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Mouse CD96 (Catalog # 5690-CD) binds with an ED50 of 1.5-7.5 ng/mL.

Formulation, Preparation and Storage

9764-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD155/PVR

CD155, also known as PVR (poliovirus receptor), Necl-5 (nectin-like molecule-5) and, in rodents, TAGE4 (tumor-associated glycoprotein E4), is a 70-kDa type I transmembrane glycoprotein in the nectin-related family of adhesion proteins within the immunoglobulin superfamily (1, 2). The protein may play a role in cancer cell invasion and migration, and binds other molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT but does not bind homotypically (3, 4). Mature rat CD155/PVR consists of three Ig-like domains and a 21-amino acid (aa) transmembrane segment. Within the ECD, rat CD155/PVR shares 44% and 73% aa sequence identity with human and mouse CD155/PVR, respectively. CD155/PVR is up-regulated on endothelial cells by IFN-gamma and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin (1, 2, 5-8). It is preferentially expressed on Th17 cells compared to Th2 cells (9), and its activation promotes the development of Th1 responses (10). On migrating cells, CD155/PVR is concentrated at the leading edge, where it binds basement membrane Vitronectin, recruits Nectin-3-expressing cells, and cooperates with PDGF and Integrin alpha v beta 3 to promote cell migration (1, 3, 11). Enhanced CD155/PVR expression in tumor cells contributes to loss of contact inhibition and increased migration but also allows tumor cell recognition and killing by DNAM-1 or CD96 expressing NK cells (1, 6, 12). Binding of monocyte DNAM-1 to endothelial cell PVR promotes transendothelial migration (7).

References

  1. Mandai, K. et al. (2015) Curr. Top. Dev. Biol. 112:197.
  2. Ravens, I. et al. (2003) Biochem. Biophys. Res. Commun. 312:1364.
  3. Sloan, K. et al. (2004) BMC Cancer. 4:73.
  4. Sato, T. et al. (2004) Genes to Cells 9:791.
  5. Escalante, N.K. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:1177.
  6. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.
  7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  8. Maier, M.K. et al. (2007) Eur. J. Immunol. 37:2214.
  9. Lozano, E. et al. (2013) J. Immunol. 191:3673.
  10. Yamashita-Kanemaru, Y. et al. (2015) J. Immunol. 194:5644.
  11. Mueller, S. and E. Wimmer (2003) J. Biol. Chem. 278:31251.
  12. Chan, C.J. et al. (2010) J. Immunol. 184:902.

Long Name

Poliovirus Receptor

Alternate Names

CD155, HVED, Necl-5, PVR, PVS

Entrez Gene IDs

5817 (Human); 52118 (Mouse); 25066 (Rat); 102136444 (Cynomolgus Monkey); 712851 (Rhesus Macaque)

Gene Symbol

PVR

UniProt

NP_058772

Additional CD155/PVR Products

Product Documents for Recombinant Rat CD155/PVR His-tag Protein, CF

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Product Specific Notices for Recombinant Rat CD155/PVR His-tag Protein, CF

For research use only

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