Recombinant Rat Fc gamma RIIA/CD32a Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9696-CD

R&D Systems, part of Bio-Techne
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9696-CD-100
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Key Product Details

Source

HEK293

Accession #

NP_446295

Conjugate

Unconjugated

Applications

Bioactivity

View all Fc gamma RIIA/CD32a Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived rat Fc gamma RIIA/CD32a protein
Asp37-His220, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp37

Predicted Molecular Mass

22 kDa

SDS-PAGE

29-44 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Rat Fc gamma RIIA/CD32a is immobilized on an anti-His Tag Antibody coated plate, it binds Biotinylated Rat IgG. The concentration of Biotinylated Rat IgG that produces 50% of the optimal binding response is 1.25-6.25 μg/mL

Scientific Data Images for Recombinant Rat Fc gamma RIIA/CD32a Protein, CF

Recombinant Rat Fc gamma RIIA/CD32a Protein Binding Activity

Recombinant Rat Fc gamma RIIA/CD32a Protein Binding Activity

When Recombinant Rat Fc gamma RIIA/CD32a (Catalog # 9696-CD) is immobilized on an anti-His Tag Antibody coated plate, it binds Biotinylated Rat IgG with an ED50 of 1.25-6.25 μg/mL.

Formulation, Preparation and Storage

9696-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIIA/CD32a

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, cytokine release, and B cell proliferation. There are three classes of Fc gamma Rs, based on close relationships in their extracellular domains: Fc gamma RI/CD64, Fc gamma RII/CD32, and Fc gamma RIII/CD16. Each group may be encoded by multiple genes and exists in different isoforms depending on species and cell type (1-3). In humans, there are three Fc gamma RII genes that encode multiple transcripts that differ in their cytoplasmic tails. Only one murine Fc gamma RII gene has been identified which has two transcripts that are homologues of the human Fc gamma RIIB (4). Fc gamma RIIA is not expressed in mice. Approximately 40 kDa, Fc gamma RIIA is a type 1 transmembrane glycoprotein that is expressed on different cell types, including, neutrophils, monocytes, dendritic cells, and is the only Fc gamma R expressed on human platelets (5). The mature protein consists of an extracellular domain (ECD), a transmembrane segment, and a cytoplasmic domain. Within the ECD, rat Fc gamma RIIA shares 52% amino acid sequence identity with human Fc gamma RIIA. The Fc gamma RI/CD64 proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the Fc gamma RII/CD32 and Fc gamma RIII/CD16 proteins bind IgG with lower affinities (~10-6-10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 6). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 7). The only inhibitory member in human and mouse, Fc gamma RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (7).

References

  1. Van de Winkel, J. and P. Capel (1993) Immunol. Today. 14:215.
  2. Raghavan, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
  3. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Ravetch, J.V. and J.P. Kinet (1991) Annu. Rev. Immunol. 9:457.
  5. Arman, M. and K. Krauel (2015) J. Thromb. Haemost. 13:893.
  6. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  7. Ravetch, J. and L. Lanier (2000) Science 290:84.

Long Name

Fc gamma Receptor II A

Alternate Names

CD32a, FCGR2A, FcgRIIA, FCRIIA

Entrez Gene IDs

2212 (Human); 14130 (Mouse); 116591 (Rat); 102143568 (Cynomolgus Monkey)

Gene Symbol

FCGR2A

UniProt

NP_446295

Additional Fc gamma RIIA/CD32a Products

Product Documents for Recombinant Rat Fc gamma RIIA/CD32a Protein, CF

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Product Specific Notices for Recombinant Rat Fc gamma RIIA/CD32a Protein, CF

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