Recombinant Rat GFR alpha-1 Fc Chimera Protein

R&D Systems, part of Bio-Techne | Catalog # 560-GR

R&D Systems, part of Bio-Techne
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Carrier Free
560-GR-100/CF
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With Carrier
560-GR-100
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Key Product Details

Source

NS0

Accession #

Q62997

Conjugate

Unconjugated

Applications

Binding Activity, Bioactivity

View all GFR alpha-1/GDNF R alpha-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat GFR alpha-1/GDNF R alpha-1 protein
Rat GFR alpha-1
(Asp25-Leu445)
Accession # Q62997		 CSIEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp25

Predicted Molecular Mass

74 kDa (monomer)

SDS-PAGE

95 kDa, reducing conditions

Activity

Measured by its ability to confer GDNF responsiveness to PC-12 rat adrenal pheochromocytoma cells using a neurite outgrowth assay. Treanor, J. et al. (1996) Nature 392:80.
The ED50 for this effect is approximately 0.3 µg/mL in the presence of 25 ng/mL of Recombinant Rat GDNF (Catalog # 512-GF).

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Rat GDNF (Catalog # 512-GF) at 1 μg/mL binds Recombinant Rat GFR alpha‑1/GDNF R alpha‑1 Fc Chimera with an apparent KD <5 nM.

Reviewed Applications

Read 2 reviews rated 4.5 using 560-GR in the following applications:

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 560-GR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 560-GR/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-1/GDNF R alpha-1

Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF‑ beta superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha- (GFR alpha-1) or GFR alpha-2). GFR alpha-1 and -2 are members of a family of at least four cysteine‑rich glycosyl-phosphatidylinositol (GPI)‑linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha-1 or GFR alpha-2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alphas released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha-1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase.

Rat GFR alpha-1 cDNA encodes a 468 amino acid (aa) residue protein with an N-terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha-1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha-1 shares 93% amino acid identity with rat GFR alpha-1. The expression of the various GFR alphas are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alphas in mediating the activities of the GDNF family of neurotrophic factors.

References

  1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
  2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha 1

Alternate Names

GDNF R alpha-1, GFR alpha1, GFRa-1, GFRA1

Entrez Gene IDs

2674 (Human); 14585 (Mouse); 25454 (Rat)

Gene Symbol

GFRA1

UniProt

Q62997

Additional GFR alpha-1/GDNF R alpha-1 Products

Product Documents for Recombinant Rat GFR alpha-1 Fc Chimera Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS 560-GR (with carrier)
Download SDS 560-GR/CF (carrier free)

Product Specific Notices for Recombinant Rat GFR alpha-1 Fc Chimera Protein

For research use only

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