Recombinant Rat IL-17E Protein

R&D Systems, part of Bio-Techne | Catalog # 8949-IL

R&D Systems, part of Bio-Techne
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Carrier Free
8949-IL-025/CF
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With Carrier
8949-IL-025
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Key Product Details

Source

HEK293

Accession #

D3ZLB1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-17E/IL-25 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived rat IL-17E/IL-25 protein
Val17-Ala169

Purity

>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val17

Predicted Molecular Mass

18 kDa

SDS-PAGE

19-25 kDa, reducing conditions

Activity

Measured by its ability to induce CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cells.
The ED50 for this effect is 0.25-1.25 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 8949-IL
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 8949-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.

Background: IL-17E/IL-25

The Interleukin-17 (IL-17) family of proteins are immunoregulatory cytokines that share a conserved cysteine-rich region. IL-17E, which is also known as IL-25, promotes Th2-biased immune responses. This is in contrast to other IL-17 family members which promote Th1- and Th17-biased inflammation. IL-25 is an important mediator of allergic reactions and protection against intestinal parasites (1, 2). Mature rat IL-25 shares 80% and 91% amino acid sequence identity with mouse and human IL-25, respectively (3, 4). During helminth infections and allergic reactions, IL-25 is locally up-regulated in intestinal and airway epithelial cells, atopic dermatitis skin lesions, and local Th2 cells, eosinophils, and basophils (4-9). It binds to IL-17 RB but also requires IL-17 RA to exert its activity (3, 8, 10). IL-25 acts on a variety of cell types which respond with increased production of Th2 cytokines (e.g. IL-4, IL-5, IL-13) and reduced production of Th1 and Th17 cytokines (e.g. IFN-gamma, IL-12, IL-23, IL-17A, IL-17F) (4-6, 8, 9, 11-15). Airway IL-25 can be activated by MMP-7, a protease that is up-regulated in airway epithelium in response to allergen exposure (16). Cleaved IL-25 shows enhanced binding to IL-17 RB and stronger induction of Th2 cytokines (16). The Th2 cytokines, in turn, trigger expansion of Th2 memory cells and anti-inflammatory M2 macrophages, increased eosinophil mobilization and activation, and dendritic cell migration (4, 6, 9, 13). These actions promote protective anti-helminth immune responses (4, 5) as well as allergic inflammation and airway hyperreactivity (11). The IL-25 induced suppression of Th1 and Th17 cytokines limits Th17 cell expansion and disease pathology in autoimmunity and colitis (12, 15). IL-25 also promotes vascular endothelial cell proliferation and assembly into tubular structures (7). It supports the integrity of the blood-brain barrier and limits CD4+ T cell infiltration into the brain (17).

References

  1. Saadoun, D. et al. (2011) Curr. Pharm. Des. 17:3781.
  2. Iwakura, Y. et al. (2011) Immunity 34:149.
  3. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
  4. Fort, M.M. et al. (2001) Immunity 15:985.
  5. Zhao, A. et al. (2010) J. Immunol. 185:6921.
  6. Suzukawa, M. et al. (2012) J. Immunol. 189:3641.
  7. Corrigan, C.J. et al. (2011) Proc. Natl. Acad. Sci. USA 108:1579.
  8. Petersen, B.C. et al. (2012) Nat. Med. 18:751.
  9. Wang, Y.-H. et al. (2007) J. Exp. Med. 204:1837.
  10. Rickel, E.A. et al. (2008) J. Immunol. 181:4299.
  11. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
  12. Kleinschek, M.A. et al. (2007) J. Exp. Med. 204:161.
  13. Cao, Q. et al. (2011) J. Am. Soc. Nephrol. 22:1229.
  14. Stock, P. et al. (2009) J. Immunol. 182:5116.
  15. Caruso, R. et al. (2009) Gastroenterology 136:2270.
  16. Goswami, S. et al. (2009) Nat. Immunol. 10:496.
  17. Sonobe, Y. et al. (2009) J. Biol. Chem. 284:31834.

Long Name

Interleukin 17E

Alternate Names

IL-25, IL17E, IL25

Entrez Gene IDs

64806 (Human); 140806 (Mouse); 501996 (Rat)

Gene Symbol

IL25

UniProt

D3ZLB1

Additional IL-17E/IL-25 Products

Product Documents for Recombinant Rat IL-17E Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS 8949-IL (with carrier)
Download SDS 8949-IL/CF (carrier free)

Product Specific Notices for Recombinant Rat IL-17E Protein

For research use only

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