Recombinant Rat MuSK Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9847-MK

R&D Systems, part of Bio-Techne
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9847-MK-050
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Key Product Details

Source

NS0

Accession #

Q62838

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all MuSK Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat MuSK protein
Rat MuSK
(Glu22-Thr494)
Accession # Q62838		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu22

Predicted Molecular Mass

79 kDa

SDS-PAGE

89-104 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
In the presence of Recombinant Rat Agrin (Catalog # 550-AG), immobilized Recombinant Rat MuSK Fc Chimera (2 µg/mL at 100 µL/well) binds Recombinant Human LRP-4 (Catalog # 5948-LR) with an ED50 of 0.06-0.3 μg/mL.

Scientific Data Images for Recombinant Rat MuSK Fc Chimera Protein, CF

Recombinant Rat MuSK Fc Chimera Protein Binding Activity

Recombinant Rat MuSK Fc Chimera Protein Binding Activity

When Recombinant Rat MuSK Fc Chimera (Catalog # 9847-MK) is coated at 2 µg/mL, Recombinant Human LRP-4 (Catalog # 5948-LR) binds with an ED50 of 0.06-0.3 µg/mL in the presence of Recombinant Rat Agrin (Catalog # 550-AG).

Formulation, Preparation and Storage

9847-MK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MuSK

MuSK (muscle-specific kinase) is a 100 kDa type I transmembrane (TM) protein belonging to the receptor tyrosine kinase family (1). Rat MuSK contains a 473 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 353 cytoplasmic domain (1). Within the ECD, rat MuSK shares 92% and 96% aa sequence identity with human MuSK and mouse MuSK, respectively. It is found in the postsynaptic membrane of skeletal muscle motor endplates (2). MuSK has multiple isoforms. One contains deletions at residues 307‑394 and 454‑461, while a second is a short soluble form that contains residues 120‑209 plus a unique 24 aa C‑terminal tail (3). MuSK binds the heparin sulfate proteoglycan agrin to promote acetylcholine receptor clustering. It has also been found to bind with low-density lipoprotein receptor-related protein 4 (LRP4) (4), Wnt ligands (5), biglycan (6), and ColQ (7). Recent studies have showed that MuSK does not bind agrin directly, but enhanced the MuSK-LRP4 interaction (7-8). When Agrin binds to the N-Terminal region of LRP4, this promotes the association of LRP4 and MuSK, which then stimulates MuSK kinase activity (9-10).

References

  1. Valenzuela, DM. et al.(1995) Neuron. 15:573.
  2. Finn, A.J. et al. (2003) Nat. Neurosci. 6:717.
  3. Nasrin, F. et al. (2014) Sci. Rep. 4:6841.
  4. Kim, N. et al. (2008) Cell 135:334.
  5. Banerjee, S. et al. (2011) Development 138:3287.
  6. Amenta, A.R. et al. (2012) J Neurosci 32:2324.
  7. Otsuka, K. et al. (2015) Scientific Reports. 5:13928.
  8. Zhang, B. et al. (2008) Neuron 60:285.
  9. Zhang, W. et al (2011) J Biol Chem. 286(47):40624.
  10. Burden, S.J. et al. (2017) CSH Perspectives Biol. 5:a009167.

Long Name

Muscle-specific Receptor Tyrosine Kinase

Alternate Names

EC 2.7.10, EC 2.7.10.1, MGC126323, MGC126324, muscle, skeletal, receptor tyrosine kinase, MuSK, skeletal receptor tyrosine-protein kinase

Entrez Gene IDs

4593 (Human); 18198 (Mouse); 81725 (Rat); 102127677 (Cynomolgus Monkey)

Gene Symbol

MUSK

UniProt

Q62838

Additional MuSK Products

Product Documents for Recombinant Rat MuSK Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Rat MuSK Fc Chimera Protein, CF

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