Recombinant Rat RAGE Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1616-RG

R&D Systems, part of Bio-Techne
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1616-RG-050
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Key Product Details

Source

NS0

Accession #

Q63495

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all RAGE/AGER Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat RAGE protein
Rat RAGE
(Gln24 - Ala342)
Accession # Q63495		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln24 predicted

Predicted Molecular Mass

60 kDa (monomer)

SDS-PAGE

80-90 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
rrRAGE/Fc Chimera immobilized at 5 µg/mL (100 µL/well) on a goat anti-human IgG Fc antibody-coated plate (0.5 µg/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA, Catalog # BT4127) with a linear range of 0.02-15 µg/mL.

Formulation, Preparation and Storage

1616-RG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RAGE/AGER

Advanced glycation endproducts (AGEs) are adducts formed by the non‑enzymatic glycation of macromolecules. AGE formation is accelerated in oxidative and hyperglycemic conditions, diabetes, renal failure, atherosclerosis, Alzheimer’s disease, arthritis, and in normal aging (1 - 5). Receptor for advanced glycation endproducts (RAGE) is a 35 kDa type I transmembrane protein belonging the immunoglobulin superfamily. Besides AGEs, RAGE binds beta-amyloid peptide, S100/calgranulin family proteins, HMGB1/amphoterin, and leukocyte integrins (6 ‑ 9). Mature rat RAGE consists of a 319 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain and two Ig-like C-type domains, a 21 aa transmembrane segment, and a 40 aa cytoplasmic domain (10). Within the ECD, rat RAGE shares 79% and 91% aa sequence identity with human and mouse RAGE, respectively. In human, soluble forms of RAGE are generated by alternate splicing and are associated with multiple disease states (11, 12). RAGE is expressed in the embryonic central nervous system and on macrophages, monocytes, smooth muscle cells, and endothelial cells (13 ‑ 15). It is upregulated in response to AGE accumulation, and its activation induces a broad proinflammatory response (6, 15). The increased production of reactive oxygen species during inflammation promotes additional AGE formation and RAGE upregulation, a cycle that exacerbates diabetic complications and inflammation-induced tissue injury (2, 4).

References

  1. Schleicher, E. and U. Friess (2007) Kidney Int. Suppl. 106:S17.
  2. Herold, K. et al. (2007) J. Leukoc. Biol. 82:204.
  3. Thornalley, P.J. (2006) J. Ren. Nutr. 16:178.
  4. Goldin, A. et al. (2006) Circulation 114:597.
  5. Ramasamy, R. et al. (2005) Glycobiology 15:16R.
  6. Kislinger, T. et al. (1999) J. Biol. Chem. 274:31740.
  7. Yan, S.D. et al. (1996) Nature 382:685.
  8. Huttenen, H. et al. (2000) J. Biol. Chem. 275:40096.
  9. Chavakis, T. et al. (2003) J. Exp. Med. 198:1507.
  10. Renard, C. et al. (1997) Mol. Pharmacol. 52:54.
  11. Yonekura H, et al. (2003) Biochem. J. 370:1097.
  12. Koyama, H. et al. (2007) Mol. Med. 13:625.
  13. Hori, O. et al. (1995) J. Biol. Chem. 270:25752.
  14. Brett, J. et al. (1993) Am. J. Pathol. 143:1699.
  15. Bierhaus, A. et al. (2006) Curr. Opin. Investig. Drugs 7:985.

Long Name

Receptor for Advanced Glycation End Products

Alternate Names

AGER, SCARJ1

Entrez Gene IDs

177 (Human); 11596 (Mouse); 81722 (Rat); 403168 (Canine)

Gene Symbol

AGER

UniProt

Q63495

Additional RAGE/AGER Products

Product Documents for Recombinant Rat RAGE Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Rat RAGE Fc Chimera Protein, CF

For research use only

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