Recombinant Rat TIMP-1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 580-RT
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived rat TIMP-1 protein
Cys24-Ala217
Cys24-Ala217
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Cys24
Predicted Molecular Mass
21.5 kDa
SDS-PAGE
32-34 kDa, reducing conditions
Activity
Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001).
The IC50 value is <3.0 nM, as measured under the described conditions.
The IC50 value is <3.0 nM, as measured under the described conditions.
Formulation, Preparation and Storage
580-RT
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution |
Reconstitute at 500 μg/mL in sterile, deionized water.
|
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TIMP-1
Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 32‑34 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.
Long Name
Tissue Inhibitors of Metalloproteinases 1
Alternate Names
TIMP1
Gene Symbol
TIMP1
UniProt
Additional TIMP-1 Products
Product Documents for Recombinant Rat TIMP-1 Protein, CF
Product Specific Notices for Recombinant Rat TIMP-1 Protein, CF
For research use only
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