Recombinant S. cerevisiae PPA1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8088-PP

R&D Systems, part of Bio-Techne
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8088-PP-050
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Key Product Details

Source

E. coli

Accession #

P00817

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Inorganic Pyrophosphatase/PPA1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived yeast Inorganic Pyrophosphatase/PPA1 protein
Thr2-Val287, with an N-terminal Met and 6-His tag

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

33 kDa

SDS-PAGE

36 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze pyrophosphate.
The specific activity is >125,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8088-PP
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and MgCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Inorganic Pyrophosphatase/PPA1

Inorganic pyrophosphatases are enzymes that catalyze the hydrolysis of pyrophosphate to two phosphate ions (1). This reaction is highly exergonic, and is utilized in many biochemical pathways, such as DNA synthesis (2) and bone formation (3), to render reactions effectively irreversible (4, 5). Likewise, inorganic pyrophosphatases can be coupled to unfavorable biochemical reactions in order to drive these reactions to completion, such as in the synthesis of activated sulfur donor 3’-phosphoadenosine-5’-phosphosulfate (PAPS) (6).

References

  1. Harold, FM (1966) Bacteriol. Rev. 30:772.
  2. Nelson, D.L. and Cox, M.M. (2000) Lehninger Principles of Biochemistry, 3rd ed. pp.937. ISBN 1-57259-153-6.
  3. Poole, K.E. and Reeve, J. (2005) Curr. Opin. Pharmacol. 5:612.
  4. Takahashi, K. et al (2004) Biochem. Biophys. Res. Commun. 325:203.
  5. Terkeltaub, R.A. (2001) Am. J. Physiol., Cell Physiol. 281:C1.
  6. Wu, Z.L. et al (2010) BMC Biotechnology 10:11.

Alternate Names

IOPPP, IPP1, PP1, PPase, SID6-8061

Entrez Gene IDs

5464 (Human); 852296 (Yeast)

Gene Symbol

PPA1

UniProt

P00817

Additional Inorganic Pyrophosphatase/PPA1 Products

Product Documents for Recombinant S. cerevisiae PPA1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant S. cerevisiae PPA1 Protein, CF

For research use only

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