Recombinant SARS-CoV-2 Membrane Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10690-CV

Extracellular Domain
R&D Systems, part of Bio-Techne
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10690-CV-100
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Key Product Details

Source

CHO

Accession #

YP_009724393.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Membrane Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived sars-cov-2 Membrane protein
SARS-CoV-2 Membrane Protein 
(Met1-Asn21)
Accession # YP_009724393.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

29 kDa

SDS-PAGE

33-43 kDa, under reducing conditions

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant SARS-CoV-2 Membrane Fc Chimera Protein, CF

Recombinant SARS-CoV-2 Membrane Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant SARS-CoV-2 Membrane Fc Chimera Protein (Catalog # 10690-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions. Visualized by Coomassie® Blue staining, showing bands at 33-43 kDa and 60-80 kDa, respectively.

Formulation, Preparation and Storage

10690-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Membrane

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). M protein is the most abundant structural protein in the coronavirus membrane, and it is predicted to span the membrane three times, with a short N-terminal domain outside the viral envelope and a long C-terminal domain inside the virion (2). SARS-CoV-2 M protein is a 222 amino acid (aa) glycoprotein that is composed of an 18 aa N-terminal domain on the viral surface, 3 transmembrane domains, and a 122 aa C-terminal domain inside the viral envelope. SARS-CoV-2 M protein shares 89.14%, 98.6%, 98.2%, and 38.36% aa similarity with SARS-CoV-1, bat SARS-CoV, pangolin SARS-CoV, and MERS-CoV M proteins, respectively (3). The M protein of coronavirus plays an important role in assembly of viral particles by interacting with other structural proteins, especially with the E protein (4, 5). In SARS-CoV-2, M protein, combined with E protein, regulates intracellular trafficking of the S Protein and its unique furin-mediated processing (6).

References

  1. Wu, F. et al. (2020) Nature 579:265.
  2. Mousavizadeh, L. and S. Ghasemi (2020) J. Microbiol. Immunol. Infect. doi:10.1016/j.jmii.2020.03.022.
  3. Thomas, S. (2020) Pathog. Immun. 5:342.
  4. Masters, P.S. (2006) Adv. Virus. Res. 66:193.
  5. Corse, E. and C.E. Machamer (2003) Virology 312:25.
  6. Boson, B. et al. (2020) bioRxiv doi:10.1101/2020.08.24.260901.

Long Name

Membrane Glycoprotein

Alternate Names

M protein

Entrez Gene IDs

43740571 (SARS-CoV-2)

Gene Symbol

M

UniProt

YP_009724393.1

Additional Membrane Products

Product Documents for Recombinant SARS-CoV-2 Membrane Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant SARS-CoV-2 Membrane Fc Chimera Protein, CF

For research use only

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