Recombinant Viral Dengue Virus 4 NS1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9444-DG

R&D Systems, part of Bio-Techne
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9444-DG-100
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Key Product Details

Source

HEK293

Accession #

P09866

Conjugate

Unconjugated

View all Dengue Virus 4 NS1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived viral Dengue Virus 4 NS1 protein
Asp775-Ala1126, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp775

Predicted Molecular Mass

40 kDa

SDS-PAGE

44-53 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9444-DG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dengue Virus 4 NS1

Dengue virus type 1 non-structural protein 1 (DENV4 NS1) is an approximately 50 kDa viral glycoprotein (1). Dengue viruses are mosquito-borne flaviviruses that exist in nature as four distinct serotypes (DENV1-4) (2). Dengue virus NS1 can be found associated with cell membranes, within cells and at the cell surface, or as secreted extracellular species. The secreted form of NS1 is known to be hexameric (1). Within cells, NS1 plays a significant role in virus replication and has been shown to co-localize with dsRNA and other components of viral replication complexes in viral-induced vesicle packets (1,3). Cell surface-associated and secreted NS1 are profoundly immunogenic, and both the protein itself and the antibodies it elicits have been shown to contribute to either protection or pathogenesis and innate immune evasion (1, 3). Mature DENV4 NS1 contains 352 amino acids (aa) and consists of 2 conserved N-glycosylation sites and 12 invariant cysteine residues that form six intramolecular disulfide bonds (3). Mature DENV4 NS1 shares 69%, 73%, and 74% aa sequence identity with DENV1, DENV2, and DENV3 NS1, respectively.

References

  1. Muller, D. et al. (2012) J. Gen. Virol. 93:771.
  2. VanBlargan, L.A. et al. (2013) PLoS Pathog. 9:e1003761.
  3. Flamand, M. et al. (1999) J. Virol. 73:6104

Long Name

Dengue Virus 4 Dominica/814669/1981 Polyprotein

Alternate Names

DEN-4, Dengue virus Serotype 4, Dengue virus Type 4, DENV4, DENV4-NS1

Entrez Gene IDs

5075729 (Viral)

Gene Symbol

POLY

UniProt

P09866

Additional Dengue Virus 4 NS1 Products

Product Documents for Recombinant Viral Dengue Virus 4 NS1 Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Viral Dengue Virus 4 NS1 Protein, CF

For research use only

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