Recombinant Viral EBV IL-10 Protein
R&D Systems, part of Bio-Techne | Catalog # 915-VL
Key Product Details
Source
E. coli
Accession #
Structure / Form
Noncovalently-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
E. coli-derived viral IL-10 protein
Gln26-Arg170, with an N-terminal Met
Gln26-Arg170, with an N-terminal Met
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
17.2 kDa
Activity
Measured in a cell proliferation assay using MC/9-2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507.
The ED50 for this effect is 3-15 ng/mL.
The ED50 for this effect is 3-15 ng/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 915-VL
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 915-VL/CF
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-10
Interleukin 10 (IL-10) is a pleiotropic cytokine that plays an important role in regulating inflammatory and immune responses. It is a potent suppressant of proinflammatory cytokine production by monocytes/macrophages and neutrophils. It is also an inhibitor of macrophage and T cell effector functions. Homologs of mammalian IL-10 have been identified in the genome of several viruses, including Epstein-Barr virus (EBV), poxvirus Orf, baboon cytomegalovirus, and human and equine herpes virus. In the EBV genome, the BCRF1 open reading frame encodes the EBV IL-10 (vIL-10). The viral IL-10 precursor is a 170 amino acid residue (aa) protein with a putative 25 aa signal peptide that is cleaved to yield the 145 aa mature protein. The EBV IL-10 precursor shares approximately 78% and 65% amino acid sequence homology with human and mouse IL-10, respectively. Most of the deviations are localized to the signal peptide and the first 20 amino-terminal residues. Viral IL-10 is expressed during the late phase of the lytic cycle of EBV infection. Viral IL-10 is a partial agonist of mammalian IL-10 and shares many of their activities. It is likely that vIL-10 may have a role in host immune evasion.
References
- Moore, K.W. et al. (1993) Annu. Rev. Immunol. 11:165.
Long Name
Interleukin 10
Alternate Names
CSIF, GVHDS, IL10, IL10A, TGIF
Entrez Gene IDs
Gene Symbol
IL10
UniProt
Additional IL-10 Products
Product Documents for Recombinant Viral EBV IL-10 Protein
Product Specific Notices for Recombinant Viral EBV IL-10 Protein
For research use only
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