Recombinant Viral RSV Active Src Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3389-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived viral Src protein
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N-terminal GST tag
SDS-PAGE
83 kDa.
Activity
The specific activity of Src is typically 99-133 nmol/min/mg using a synthetic peptide substrate (KVEKIGEGTYGVVYK).
Scientific Data Images for Recombinant Viral RSV Active Src Protein, CF
Recombinant Viral RSV Active Src Protein SDS-PAGE.
The approximate molecular weight is 83 kDa and the purity is > 95%.Formulation, Preparation and Storage
3389-KS
| Formulation | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, and 25% Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: Src
The non-receptor tyrosine kinase Src was originally identified as a transforming protein of the Rous sarcoma virus that had enzymatic ability to phosphorylate tyrosine in protein substrates (1). The proto-oncogene c-Src is the cellular homologue of viral Src (v-Src) and the founding member of the Src family kinases. c-Src is over-expressed and activated in a large number of human maligancies and has been linked to the development of cancer and progression to distant metastases (2). In addition to increasing cell proliferation, a key role of c-Src in cancer seems to be the ability to promote invasion and motility, functions that might contribute to tumor progression.
Although v-Src and c-Src share 88% amino acid identity, v-Src, unlike c-Src, is constitutively active mainly because it lacks a crucial c-terminal negative-regulatory region (3). As a result, v-Src is missing a tyrosine residue (Y530 in human c-Src) that upon phosphorylation contributes to c-Src assuming an inactive conformation.
References
- Collett, M.S. et al. (1978) Proc. Natl. Acad. Sci. USA 75:2021.
- Jacobs, C. et al. (1983) Cancer Res. 43:1696.
- Yeatman, T.J. (2004) Nat. Rev. Cancer 4:470.
Long Name
v-src Sarcoma [Schmidt-Ruppin A-2] Viral Oncogene Homolog
Alternate Names
ASV, c-Src, RSVgp4
Gene Symbol
SRC
UniProt
Additional Src Products
Product Documents for Recombinant Viral RSV Active Src Protein, CF
Product Specific Notices for Recombinant Viral RSV Active Src Protein, CF
For research use only
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