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RFT1 Recombinant Protein Antigen

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-56931PEP

Novus Biologicals, part of Bio-Techne
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NBP2-56931PEP

Key Product Details

Source

E. coli

Tag

N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

Conjugate

Unconjugated

Applications

Antibody Competition

Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human RFT1.

Source: E. coli

Amino Acid Sequence: QSLCFIHRYYRRSPHRPLAGLHLSP

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-56931.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation and Storage

NBP2-56931PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: RFT1

RFT1, also known as Protein RFT1 homolog, is a 60kDa 541 amino acid protein found in the membrane. RFT1 is believed to play a role in N-linked oligosaccharide assembly. Research is being conducted on RFT1 in relationship to congenital disorder of glycosylation and hypotonia. RFT1 has been linked to the asparagine N-linked glycosylation, metabolism of proteins and post-translational protein modification pathways where it interacts with IDI1, PSMA6, ACTB, UBC and GTF2B.

Alternate Names

DKFZp667J092, FLJ25945, putative endoplasmic reticulum multispan transmembrane protein, requiring fifty three 1 homolog (S. cerevisiae), RFT1 homolog (S. cerevisiae), RFT1, requiring fifty three 1 homolog

Gene Symbol

RFT1

Additional RFT1 Products

Product Documents for RFT1 Recombinant Protein Antigen

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RFT1 Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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