Recombinant Human RhoG His Protein

Name: Recombinant Human RhoG His Protein
Brand: Novus Biologicals
SKU: NBP1-98934

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-98934

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all RhoG Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-188 of Human RhoG

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMQS IKCVVVGDGA VGKTCLLICY TTNAFPKEYI PTVFDNYSAQ SAVDGRTVNL NLWDTAGQEE YDRLRTLSYP QTNVFVICFS IASPPSYENV RHKWHPEVCH HCPDVPILLV GTKKDLRAQP DTLRRLKEQG QAPITPQQGQ ALAKQIHAVR YLECSALQQD GVKEVFAEAV RAVLNPTPIK RGRSC

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

25.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human RhoG His Protein

SDS-PAGE: Recombinant Human RhoG His Protein [NBP1-98934]

SDS-Page: Recombinant Human RhoG Protein [NBP1-98934] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-98934

Formulation	20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 50 mM Imidazole, 0.5 M NaCl
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RhoG

RhoG(Ras homology Growth-related) is a member of the Rac subfamily of the Rho family of small G proteins. RhoG is a small monomeric GTP-binding protein (G protein), and is an important component of many intracellular signalling pathways. This protien are required for the formation of membrane ruffles during macropinocytosis. It plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. Like most small G proteins RhoG is involved in a diverse set of cellular signalling mechanisms. In mammalian cells these include cell motility, gene transcription, endocytosis, neurite outgrowth, protection from anoikis and regulation of the neutrophil NADPH oxidase. Recombinant human RhoG protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

ARHGMGC125835, MGC125836, ras homolog gene family, member G (rho G), RhoG, rho-related GTP-binding protein RhoG

Gene Symbol

RHOG

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COA

Product Specific Notices for Recombinant Human RhoG His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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