Is this product carrier free?

Name: Recombinant Human RNASE3 His Protein
Brand: Novus Biologicals
SKU: NBP2-23415

This protein does not contain any carrier proteins. The buffer is as stated: 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea and 10% glycerol.

Recombinant Human RNASE3 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23415

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

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Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 28-160 of Human RNASE3

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRP PQFTRAQWFA IQHISLNPPR CTIAMRAINN YRWRCKNQNT FLRTTFANVV NVCGNQSIRC PHNRTLNNCH RSRFRVPLLH CDLINPGAQN ISNCRYADRP GRRFYVVACD NRDPRDSPRY PVVPVHLDTT I

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

19.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human RNASE3 His Protein

SDS-PAGE: Recombinant Human RNASE3 His Protein [NBP2-23415]

SDS-Page: Recombinant Human RNASE3 Protein [NBP2-23415]

Formulation, Preparation and Storage

NBP2-23415

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNase3

RNASE3 is cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. It possesses a wide variety of biological activities. This protein exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains and promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. Recombinant human RNASE3 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

Ribonuclease A Family Member 3

Alternate Names

ECP, RNS3

Gene Symbol

RNASE3

Additional RNase3 Products

Product Documents for Recombinant Human RNASE3 His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human RNASE3 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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