Recombinant Human RYBP/DEDAF His Protein

Name: Recombinant Human RYBP/DEDAF His Protein
Brand: Novus Biologicals
SKU: NBP1-98929

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-98929

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all RYBP/DEDAF Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-228 of Human RYBP/DEDAF

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMTMGDK KSPTRPKRQA KPAADEGFWD CSVCTFRNSA EAFKCSICDV RKGTSTRKPR INSQLVAQQV AQQYATPPPP KKEKKEKVEK QDKEKPEKDK EISPSVTKKN TNKKTKPKSD ILKDPPSEAN SIQSANATTK TSETNHTSRP RLKNVDRSTA QQLAVTVGNV TVIITDFKEK TRSSSTSSST VTSSAGSEQQ NQSSSGSEST DKGSSRSSTP KGDMSAVNDE SF

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

27.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human RYBP/DEDAF His Protein

SDS-PAGE: Recombinant Human RYBP/DEDAF His Protein [NBP1-98929]

SDS-Page: Recombinant Human RYBP/DEDAF Protein [NBP1-98929] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-98929

Formulation	20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT, 100 mM NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RYBP/DEDAF

RYBP (RING1- and YY1-binding protein) is a member of the polycomb group (PcG). This protein interacts with MDM2 and decreases MDM2-mediated p53 ubiquitination, leading to stabilization of p53 and an increase in p53 activity. RYBP induces cell-cycle arrest and is involved in the p53 response to DNA damage. RYBP has been shown to interact with RING1, YY1, Caspase 10, E2F3, E2F2, Mdm2, Abl gene and CBX2. Recombinant human RYBP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

AAP1, APAP-1, Apoptin-associating protein 1, Death effector domain-associated factor, DEDAFYY1 and E4TF1-associated factor 1, RING1 and YY1 binding protein, RING1 and YY1-binding protein, ring1 interactor RYBP, YEAF1DED-associated factor, YY1 and E4TF1 associated factor 1

Gene Symbol

RYBP

Additional RYBP/DEDAF Products

Product Documents for Recombinant Human RYBP/DEDAF His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human RYBP/DEDAF His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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