Recombinant Canine SCF/c-kit Ligand Protein

Name: Recombinant Canine SCF/c-kit Ligand Protein
Brand: Novus Biologicals
SKU: NBP2-34912

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34912

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Product Specifications
Scientific Data
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Key Product Details

Source

E. coli

Accession #

Q06220

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all SCF/c-kit Ligand Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 165 amino acids corresponding to SCF/c-kit Ligand Source: E. coli

Uniprot ID: Q06220

Amino Acid Sequence: KGICGKRVTD DVKDVTKLVA NLPKDYKIAL KYVPGMDVLP SHCWISVMVE QLSVSLTDLL DKFSNISEGL SNYSIIDKLV KIVDDLVECT EGYSFENVKK APKSPELRLF TPEEFFRIFN RSIDAFKDLE TVASKSSECV VSSTLSPDKD SRVSVTKPFM LPPVA

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of SCF/c-kit Ligand as determined by LAL method.

Predicted Molecular Mass

18.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Canine SCF/c-kit Ligand Protein

SDS-PAGE: Recombinant Canine SCF/c-kit Ligand Protein [NBP2-34912]

SDS-Page: Canine SCF/c-kit Ligand Protein [NBP2-34912]

Formulation, Preparation and Storage

NBP2-34912

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4. x
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: SCF/c-kit Ligand

Stem Cell Factor (SCF) which binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane SCF is required for normal hematopoietic function. SCF plays an important role in hematopoiesis, spermatogenesis and melanogenesis and it promotes mast cell adhesion, migration, proliferation, and survival. Soluble canine SCF shares 88 %, 93 %, 86 %, 83 %, 76 %, 76 %, 86 % and 88 % a.a. sequence identity with porcine, feline, bovine, human, mouse, rat, goat and equine SCF, respectively. Cells known to express SCF include endothelial cells, fibroblasts and keratinocytes .

Long Name

Stem Cell Factor

Alternate Names

c-kit Ligand, DCUA, DFNA69, FPH2, FPHH, KITLG, KL-1, MGF, SHEP7, SLF

Gene Symbol

KITLG

UniProt