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Recombinant Human SF3B14 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50997

Novus Biologicals, part of Bio-Techne
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NBP1-50997-0.05mg
NBP1-50997-0.25mg

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-125 of Human SF3B14

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEEMYDIFG KYGPIRQIRV GNTPETRGTA YVVYEDIFDA KNACDHLSGF NVCNRYLVVL YYNANRAFQK MDTKKKEEQL KLLKEKYGIN TDPPK

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

16.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SF3B14 His Protein

SDS-PAGE: Recombinant Human SF3B14 His Protein [NBP1-50997]

SDS-PAGE: Recombinant Human SF3B14 His Protein [NBP1-50997]

SDS-Page: Recombinant Human SF3B14 Protein [NBP1-50997] - 15 % SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-50997
Formulation 20 mM Tris-HCl buffer (pH8.0), 0.2 M NaCl, 5mM DTT, 1 mM EDTA, 30% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SF3B14

SF3B14, also known as SAP14, is a 125 amino acid nuclear protein that is a component of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. Required for the splicing of pre-mRNA, SF3B14 enters the spliceosome and associates with the pre-mRNA branch site facilitating the interaction of snRNP with the branch sites of U2 and U12 of the 17S U2 and the 18S U11/U12 snRNP complex. Recombinant human SF3B14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

HSPC175, Ht006, P14, pre-mRNA branch site protein p14, SAP14, SF3b 14 kDa subunit, SF3B14a, spliceosome-associated protein, 14 kDa subunit, splicing factor 3B, 14 kDa subunit

Gene Symbol

SF3B6

Additional SF3B14 Products

Product Documents for Recombinant Human SF3B14 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human SF3B14 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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