Recombinant Human SNF8 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-98935

Novus Biologicals, part of Bio-Techne
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NBP1-98935-100ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all SNF8 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-258 of Human SNF8

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMHRRGV GAGAIAKKKL AEAKYKERGT VLAEDQLAQM SKQLDMFKTN LEEFASKHKQ EIRKNPEFRV QFQDMCATIG VDPLASGKGF WSEMLGVGDF YYELGVQIIE VCLALKHRNG GLITLEELHQ QVLKGRGKFA QDVSQDDLIR AIKKLKALGT GFGIIPVGGT YLIQSVPAEL NMDHTVVLQL AEKNGYVTVS EIKASLKWET ERARQVLEHL LKEGLAWLDL QAPGEAHYWL PALFTDLYSQ EITAEEAREA LP

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

31.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SNF8 His Protein

SDS-PAGE: Recombinant Human SNF8 His Protein [NBP1-98935]

SDS-PAGE: Recombinant Human SNF8 His Protein [NBP1-98935]

SDS-Page: Recombinant Human SNF8 His Protein [NBP1-98935] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-98935
Formulation 20 mM Tris-HCl buffer (pH8.0), 50% glycerol, 2 mM DTT, 200 mM NaCl
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SNF8

SNF 8, also known as EAP30 (ELL-associated protein of 30 kDa), is localizes to both the nucleus and the cytoplasm and is a member of the SNF8 family of vacuolar sorting proteins. This protein is subunit of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Recombinant human SNF8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

EAP30 subunit of ELL complex, EAP30hVps22, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22, SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae), vacuolar-sorting protein SNF8, VPS22Dot3

Gene Symbol

SNF8

Additional SNF8 Products

Product Documents for Recombinant Human SNF8 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human SNF8 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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