Recombinant Human SPARC His Protein

Name: Recombinant Human SPARC His Protein
Brand: Novus Biologicals
SKU: NBP2-35270

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35270

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Product Specifications
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

P09486

Tag

His

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all SPARC Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 314 amino acids corresponding to SPAR, with expression vector sequence containing 6x His tag Source: E. coli

Uniprot ID: P09486

Amino Acid Sequence: MSYYHHHHHH DYDIPTTENL YFQGAMGSAP QQEALPDETE VVEETVAEVT EVSVGANPVQ VEVGEFDDGA EETEEEVVAE NPCQNHHCKH GKVCELDENN TPMCVCQDPT SCPAPIGEFE KVCSNDNKTF DSSCHFFATK CTLEGTKKGH KLHLDYIGPC KYIPPCLDSE LTEFPLRMRD WLKNVLVTLY ERDEDNNLLT EKQKLRVKKI HENEKRLEAG DHPVELLARD FEKNYNMYIF PVHWQFGQLD QHPIDGYLSH TELAPLRAPL IPMEHCTTRF FETCDLDNDK YIALDEWAGC FGIKQKDIDK DLVI

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of SPARC as determined by LAL method.

Predicted Molecular Mass

36.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

SPARC protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 ug/mL, corresponding to a specific activity of > 333 IU/mg

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35270

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: SPARC

SPARC (secreted protein, acidic and rich in cysteine). SPARC is produced by fibroblasts, capillary endothelial cells, platelets, macrophages, bone, and many tumor cells. It inhibits cell adhesion, spreading and proliferation, promotes collagen matrix formation and osteoblast differentiation, and binds and sequesters PDGF and VEGF. SPARC can be cleaved by metalloproteinases, producing a copper binding angiogenic peptide. SPARC is bound and internalized by Stabilin-1 on alternatively activated macrophages.

Long Name

Secreted Protein Acidic and Rich in Cysteine

Alternate Names

BM-40, Osteonectin

Gene Symbol

SPARC

UniProt