Streptavidin Native Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29370
Key Product Details
Accession #
Conjugate
HRP
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Product Specifications
Description
Native Bacteria Streptavidin
Source: Chromatographically purified streptavidin (Accession #:P22629)
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Source: Chromatographically purified streptavidin (Accession #:P22629)
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Purity
Chromatography
Applications
ELISA (1:20000 - 1:200000)
Immunohistochemistry (1:1000 - 1:5000)
Western Blot (1:10000 - 1:40000)
Immunohistochemistry (1:1000 - 1:5000)
Western Blot (1:10000 - 1:40000)
Application Notes
This product has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. This product can be utilized in Immunohistochemistry, Immunofluorescence, immuno-EM, Western Blotting, and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Protein / Peptide Type
Native Protein
Scientific Data Images for Streptavidin Native Protein
![Western Blot: Streptavidin Native Protein [NBP2-29370]](https://resources.bio-techne.com/images/products/Streptavidin-Full-length-Protein-Western-Blot-NBP2-29370-img0002.jpg "Western Blot: Streptavidin Native Protein [NBP2-29370]")
Western Blot: Streptavidin Native Protein [NBP2-29370]
Western Blot: Streptavidin Full length Protein [NBP2-29370] - Lane 1: Human IL-7. Lane 2: none. Load: 50 ng per lane. Primary antibody: Human IL-7 Biotin Conjugated antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase Conjugated Streptavidin at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-7. Other band(s): none.Formulation, Preparation and Storage
NBP2-29370
| Preparation Method | This product was prepared from chromatographically purified streptavidin. Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin. |
| Formulation | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Gentamicin Sulfate |
| Concentration | Lyoph |
| Reconstitution | Reconstitute with 1.0 ml deionized water. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
Background: Streptavidin
Alternate Names
SA V1; SA V2; Streptavidin V1; Streptavidin V2
UniProt
Additional Streptavidin Products
Product Documents for Streptavidin Native Protein
Product Specific Notices for Streptavidin Native Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...