Recombinant Human Survivin (Calmodulin Tag) Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBC1-18508
Key Product Details
Source
E. coli
Tag
(Calmodulin Tag)
Conjugate
Unconjugated
Applications
SDS-PAGE
Product Specifications
Description
A recombinant protein with a N-Terminal CaM-tag and corresponding to the amino acids 1-142 of Human Survivin
Source: E.coli
Amino Acid Sequence: MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAKG SHMGAPTLPP AWQPFLKDHR ISTFKNWPFL EGCACTPERM AEAGFIHCPT ENEPDLAQCF FCFKELEGWE PDDDPIEEHK KHSSGCAFLS VKKQFEELTL GEFLKLDRER AKNKIAKETN NKKKEFEETA KKVRRAIEQL AAMD
Purity
>90%, by SDS-PAGE
Endotoxin Level
< 1 EU per 1ug of protein (determined by LAL method)
Predicted Molecular Mass
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human Survivin (Calmodulin Tag) Protein
![SDS-PAGE: Recombinant Human Survivin Calmodulin Protein [NBC1-18508]](https://resources.bio-techne.com/images/products/Recombinant-Human-Survivin-Calmodulin-Protein-SDS-Page-NBC1-18508-img0001.jpg "SDS-PAGE: Recombinant Human Survivin Calmodulin Protein [NBC1-18508]")
SDS-PAGE: Recombinant Human Survivin Calmodulin Protein [NBC1-18508]
SDS-Page: Recombinant Human Survivin Calmodulin Protein [NBC1-18508] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.Formulation, Preparation and Storage
NBC1-18508
| Formulation | 20mM Tris-HCl buffer (pH 7.5), 0.1M NaCl |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: Survivin
Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).
Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).
/>References
/> 1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993
2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829
3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431
Alternate Names
API4, BIRC5
Gene Symbol
BIRC5
Additional Survivin Products
Product Documents for Recombinant Human Survivin (Calmodulin Tag) Protein
Product Specific Notices for Recombinant Human Survivin (Calmodulin Tag) Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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