Recombinant Human Tau Active, (K18), P301L Mutant, Pre-formed Fibrils Protein

Name: Recombinant Human Tau Active, (K18), P301L Mutant, Pre-formed Fibrils Protein
Brand: Novus Biologicals
SKU: NBP2-76793

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76793

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Microscopy, Functional Assay, SDS-PAGE, Western Blot

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Product Specifications

Description

An un-tagged partial Human Recombinant Tau Protein Pre-Formed Fibrils, Accession #: P10636

Source: E. coli

Amino Acid Sequence:
SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVLGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE

Purity

Ion exchange chromatography

Predicted Molecular Mass

15.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when active tau PFFs are combined with active tau monomers.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images

SDS-PAGE: Recombinant Human Tau Active, (K18), P301L Mutant, Pre-formed Fibrils Protein [NBP2-76793]

SDS-Page: Recombinant Human Tau Active, (K18), P301L Mutant, Pre-formed Fibrils Protein [NBP2-76793] - SDS-PAGE of ~15 kDa Active Human Tau Protein K18 P301L Preformed Fibrils (NBP2-76793). Lane 1: MW Ladder. Lane 2: Tau Protein Preformed Fibrils (NBP2-76793).

In vitro assay: Recombinant Human Tau Active, (K18), P301L Mutant, Pre-formed Fibrils Protein [NBP2-76793]

In vitro assay: Recombinant Human Tau Active, (K18), P301L Mutant, Pre-formed Fibrils Protein [NBP2-76793] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau Pre-formed Fibrils (NBP2-76793). Tau Pre-formed Fibrils (NBP2-76793) seed the formation of new tau fibrils when combined with tau monomers . Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.

Electron Microscopy: Recombinant Human Tau Active, (K18), P301L Mutant, Pre-formed Fibrils Protein [NBP2-76793] - TEM of recombinant Tau (K18), P301L mutant Pre-formed Fibrils (PFFs) at 150kx magnification. HV=80kV.

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Formulation, Preparation and Storage

NBP2-76793

Formulation	10 mM HEPES (pH 7.4), 100 mM NaCl
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: Tau

Tau is a microtubule binding protein that promotes microtubule assembly and stability. Tau is found to be the major component of the paired helical filaments (PHFs) found in the brains of patients with Alzheimer disease (AD) (1,2). Tau is hyperphosphorylated in PHFs, and specific phosphorylation sites have been implicated in the loss of Tau's association with the membrane cortex during AD disease state, including Ser 199/202, Thr 231, and Ser 393/404 (3). Glycogen synthase kinase-3, or GSK-3, phosphorylates Tau on Ser 396 (4).

Long Name

Microtubule-Associated Protein Tau

Alternate Names

MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND

Gene Symbol

MAPT

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COA

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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