Recombinant Human TFF3 Protein

Name: Recombinant Human TFF3 Protein
Brand: Novus Biologicals
SKU: NBP2-35060

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35060

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

Q07654

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all TFF3 Proteins and Enzymes »

Product Specifications

Description

A homodimeric protein consisting of two 59 amino acid chains corresponding to TFF3, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds Source: E. coli

Uniprot ID: Q07654

Amino Acid Sequence: EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of TFF3 as determined by LAL method.

Predicted Molecular Mass

13.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Trefoil Factor 3 protein is fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 ug/ml, corresponding to a specific activity of > 100 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TFF3 Protein

SDS-PAGE: Recombinant Human TFF3 Protein [NBP2-35060]

SDS-Page: Human TFF3 Protein [NBP2-35060]

Formulation, Preparation and Storage

NBP2-35060

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: TFF3

Trefoil Factor 3 (TFF3), also known as ITF and P1.B, plays an important role in epithelial regeneration and wound healing. It is expressed by epithelial goblet cells in the respiratory tract, biliary and breast ducts, small and large intestine, and cardia of the stomach. It is upregulated in response to a range of gastrointestinal epithelial disruptions and induces the migration of biliary, bronchial, and intestinal epithelial cells. Its upregulation is also associated with and enhances tumor cell invasion and metastasis. TFF3 promotes angiogenesis and enhances VEGF upregulation in tumor cells. It can form disulfide-linked dimers or associate into disulfide-linked complexes with other intestinal mucous proteins including FCGBP and MUC-2.

Long Name

Trefoil Factor 3

Alternate Names

ITF, TFI

Gene Symbol

TFF3

UniProt