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Recombinant E. coli Thioredoxin-1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44476

Novus Biologicals, part of Bio-Techne
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NBP1-44476

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 2-109 of E.coli Thioredoxin

Source: E.coli

Amino Acid Sequence: MHHHHHHMGS DKIIHLTDDS FDTDVLKADG AILVDFWAEW CGPCKMIAPI LDEIADEYQG KLTVAKLNID QNPGTAPKYG IRGIPTLLLF KNGEVAATKV GALSKGQLKE FLDANLA

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

12.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is >70 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli Thioredoxin-1 His Protein

SDS-PAGE: Recombinant E. coli Thioredoxin-1 His Protein [NBP1-44476]

SDS-PAGE: Recombinant E. coli Thioredoxin-1 His Protein [NBP1-44476]

SDS-Page: Recombinant E. coli Thioredoxin Protein [NBP1-44476] - 115% SDS-Page (3ug)

Formulation, Preparation and Storage

NBP1-44476
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thioredoxin-1

Thioredoxin 1(Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant E.coli TXN protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Thioredoxin1, Trx1, TXN, TXN1

Gene Symbol

TXN

Additional Thioredoxin-1 Products

Product Documents for Recombinant E. coli Thioredoxin-1 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant E. coli Thioredoxin-1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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