Recombinant Human Thioredoxin Reductase 1/TRXR1 His Protein

Name: Recombinant Human Thioredoxin Reductase 1/TRXR1 His Protein
Brand: Novus Biologicals
SKU: NBP1-50928

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50928

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all Thioredoxin Reductase 1/TRXR1 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 161-649 of Human Thioredoxin Reductase 1/TRXR1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MYDYDLIIIG GGSGGLAAAK EAAQYGKKVM VLDFVTPTPL GTRWGLGGTC VNVGCIPKKL MHQAALLGQA LQDSRNYGWK VEETVKHDWD RMIEAVQNHI GSLNWGYRVA LREKKVVYEN AYGQFIGPHR IKATNNKGKE KIYSAERFLI ATGERPRYLG IPGDKEYCIS SDDLFSLPYC PGKTLVVGAS YVALECAGFL AGIGLDVTVM VRSILLRGFD QDMANKIGEH MEEHGIKFIR QFVPIKVEQI EAGTPGRLRV VAQSTNSEEI IEGEYNTVML AIGRDACTRK IGLETVGVKI NEKTGKIPVT DEEQTNVPYI YAIGDILEDK VELTPVAIQA GRLLAQRLYA GSTVKCDYEN VPTTVFTPLE YGACGLSEEK AVEKFGEENI EVYHSYFWPL EWTIPSRDNN KCYAKIICNT KDNERVVGFH VLGPNAGEVT QGFAAALKCG LTKKQLDSTI GIHPVCAEVF TTLSVTKRSG ASILQAGCCG

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

55.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 15 units/mg, and was measured in a coupled assay with 5,5 -Dithiobis(2-nitrobenzoic acid) (DTNB) and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Thioredoxin Reductase 1/TRXR1 His Protein

SDS-PAGE: Recombinant Human Thioredoxin Reductase 1/TRXR1 His Protein [NBP1-50928]

SDS-Page: Recombinant Human Thioredoxin Reductase 1/TRXR1 Protein [NBP1-50928] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation and Storage

NBP1-50928

Formulation	20mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.1mM PMSF, 0.1M NaCl
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thioredoxin Reductase 1/TRXR1

TXNRD1, also known as Thioredoxin reductase 1, is a part of a selenium-containing pyridine nucleotide-disulphide oxidoreductase family, which has a conserved catalytic site of Cys-Val-Asn-Val-Gly-Cys. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. Inhibition of TXNRD1 activity may provide for potential treatments of cancer, AIDS and other autoimmune diseases as well as bacterial infections and parasitic diseases. Recombinant human TXNRD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

GRIM-12, KDRF, TR1, TRXR1, TRXRD1, TXNR, TXNRD1

Gene Symbol

TXNRD1

Additional Thioredoxin Reductase 1/TRXR1 Products

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COA

Product Specific Notices for Recombinant Human Thioredoxin Reductase 1/TRXR1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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