Recombinant Human TL1A/TNFSF15 Protein

Name: Recombinant Human TL1A/TNFSF15 Protein
Brand: Novus Biologicals
SKU: NBP2-35067

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35067

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

O95150

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all TL1A/TNFSF15 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 180 amino acids corresponding to TL1A/TNFSF15 Source: E. coli

Uniprot ID: O95150

Amino Acid Sequence: LKGQEFAPSH QQVYAPLRAD GDKPRAHLTV VRQTPTQHFK NQFPALHWEH ELGLAFTKNR MNYTNKFLLI PESGDYFIYS QVTFRGMTSE CSEIRQAGRP NKPDSITVVI TKVTDSYPEP TQLLMGTKSV CEVGSNWFQP IYLGAMFSLQ EGDKLMVNVS DISLVDYTKE DKTFFGAFLL

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of TL1A/TNFSF15 as determined by LAL method.

Predicted Molecular Mass

20.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

TL1A/TNFSF15 protein is fully biologically active when compared to standard. The ED50 as determined by its ability to induce apoptosis using human TF-1 cells is less than 20 ng/ml, corresponding to a specific activity of > 5.0 x 10^4 IU/mg

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TL1A/TNFSF15 Protein

SDS-PAGE: Recombinant Human TL1A/TNFSF15 Protein [NBP2-35067]

SDS-Page: Recombinant Human TL1A/TNFSF15 Protein [NBP2-35067]

Formulation, Preparation and Storage

NBP2-35067

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, with 0.02% Tween-20.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: TL1A/TNFSF15

TL-1A is a type II transmembrane protein belonging to the TNF superfamily and has been designated TNF superfamily member 15 (TNFSF15). TL-1A is predominantly expressed in endothelial cells and its expression is inducible by TNF-alpha and IL-1alpha. TL- 1A binds with high affinity to death receptor 3 (DR3), known as TNFRSF25. Depending on the cell context, ligation of DR3 by TL-1A can trigger one of two signaling pathways, activation of the transcription factor NF-kB, or activation of caspases and apoptosis. Recombinant human TL-1A is a 20.5 kDa polypeptide of 180 amino acid residues.

Long Name

TNF-like 1

Alternate Names

TNFSF15, VEGI, TL-1A, TL-1A/TNFSF15

Gene Symbol

TNFSF15

UniProt