Recombinant Human TNF RI/TNFRSF1A Protein

Name: Recombinant Human TNF RI/TNFRSF1A Protein
Brand: Novus Biologicals
SKU: NBP2-35274

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35274

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P19438

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all TNF RI/TNFRSF1A Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 190 amino acids corresponding to TNF RI/TNFRSF1A Source: E. coli

Uniprot ID: P19438

Amino Acid Sequence: IYPSGVIGLV PHLGDREKRD SVCPQGKYIH PQNNSICCTK CHKGTYLYND CPGPGQDTDC RECESGSFTA SENHLRHCLS CSKCRKEMGQ VEISSCTVDR DTVCGCRKNQ YRHYWSENLF QCFNCSLCLN GTVHLSCQEK QNTVCTCHAG FFLRENECVS CSNCKKSLEC TKLCLPQIEN VKGTEDSGTT

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of TNF RI/TNFRSF1A as determined by LAL method.

Predicted Molecular Mass

21.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

TNF RI/TNFRSF1A protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 cells is less than 0.05 ug/ml, corresponding to a specific activity of > 2 x 10^4 IU/mg in the presence of 0.25 ng/mL of rHuTNF-alpha.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TNF RI/TNFRSF1A Protein

SDS-PAGE: Recombinant Human TNF RI/TNFRSF1A Protein [NBP2-35274]

SDS-Page: Recombinant Human TNF RI/TNFRSF1A Protein [NBP2-35274]

Formulation, Preparation and Storage

NBP2-35274

Formulation	Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: TNF RI/TNFRSF1A

TNF Receptor I (TNF RI), also named as TNFR-I, TNF R1, TNFRSF1A, p55 and p60, belongs to the tumor necrosis factor receptor superfamily, and it is encoded by the TNFRSF1A gene in human. TNF Receptor I (TNF RI)is a universally expressed protein and is thought to form homotrimers after ligand binding. TNF Receptor I (TNF RI) is one of the major receptors for the TNF-alpha. This receptor can activate the transcription factor NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Mature human soluble TNF RI shares 68 % ~ 73 % amino acid sequence identity with murine, rat, bovine and porcine soluble TNF RI.

Long Name

Tumor Necrosis Factor Receptor I

Alternate Names

CD120a, TNFRI, TNFRSF1A, sTNF RI, sTNF RI/TNFRSF1A

Gene Symbol

TNFRSF1A

UniProt